Leukemia Inhibitory Factor and Fibroblast Growth Factor 2 Critically and Mutually Sustain Pluripotency of Rabbit Embryonic Stem Cells

• Published in: Cell transplantation Vol. 24; no. 3; pp. 319 - 338
• Main Authors: Lo, Neng-Wen, Intawicha, Payungsuk, Chiu, Yung-Tsung, Lee, Kun-Hsiung, Lu, Hsi-Chi, Chen, Chien-Hong, Chang, Yong-Hsuan, Chen, Chun-Da, Ju, Jyh-Cherng
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.01.2015; SAGE Publishing
• Subjects: Animals; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Embryonic Stem Cells - cytology; Embryonic Stem Cells - drug effects; Embryonic Stem Cells - metabolism; Fibroblast Growth Factor 2 - pharmacology; Homeodomain Proteins - metabolism; Leukemia Inhibitory Factor - pharmacology; Mice; Mitogen-Activated Protein Kinase 1 - antagonists & inhibitors; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - antagonists & inhibitors; Mitogen-Activated Protein Kinase 3 - metabolism; Octamer Transcription Factor-3 - metabolism; Phosphatidylinositol 3-Kinases - antagonists & inhibitors; Phosphatidylinositol 3-Kinases - metabolism; Phosphorylation - drug effects; Protein Kinase Inhibitors - pharmacology; Proto-Oncogene Proteins c-akt - antagonists & inhibitors; Proto-Oncogene Proteins c-akt - metabolism; Rabbits; Signal Transduction - drug effects; STAT3 Transcription Factor - metabolism; Zygote - cytology; Fibroblast growth factor 2 (FGF2); Leukemia inhibitory factor (LIF); Self-renewal; Rabbit embryonic stem cells (rESCs)

Effects of leukemia inhibitory factor (LIF) and fibroblast growth factor 2 (FGF2) on establishment and maintenance of rabbit embryonic stem cell (rESC) lines were assessed. When grown on MEF feeders, rESC lines derived from fertilized embryos were established and maintained in medium containing para...