Rapid measurement of SARS-CoV-2 spike T cells in whole blood from vaccinated and naturally infected individuals

• Published in: The Journal of clinical investigation Vol. 131; no. 17; pp. 1 - 13
• Main Authors: Tan, Anthony T, Lim, Joey Me, Le Bert, Nina, Kunasegaran, Kamini, Chia, Adeline, Qui, Martin Dc, Tan, Nicole, Chia, Wan Ni, de Alwis, Ruklanthi, Ying, Ding, Sim, Jean Xy, Ooi, Eng Eong, Wang, Lin-Fa, Chen, Mark I-Cheng, Young, Barnaby E, Hsu, Li Yang, Low, Jenny Gh, Lye, David C, Bertoletti, Antonio
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 01.09.2021
• Subjects: Adult; Antibodies; Antigens; Asymptomatic; Biomedical research; BNT162 Vaccine; Cell activation; Coronaviruses; COVID-19; COVID-19 - blood; COVID-19 - immunology; COVID-19 - prevention & control; COVID-19 vaccines; COVID-19 Vaccines - administration & dosage; Cytokines; Enzyme-linked immunosorbent assay; Female; Humans; Humoral immunity; Immunity, Cellular - drug effects; Infections; Interleukin 2; Laboratories; Lymphocytes; Lymphocytes T; Male; Middle Aged; Pandemics; Peptides; Proteins; SARS-CoV-2 - immunology; SARS-CoV-2 - metabolism; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - blood; Spike Glycoprotein, Coronavirus - immunology; Spike protein; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Vaccination; Viral infections; γ-Interferon; COVID-19; Adaptive immunity; Immunology; Cytokines; T cells

Defining the correlates of protection necessary to manage the COVID-19 pandemic requires the analysis of both antibody and T cell parameters, but the complexity of traditional tests limits virus-specific T cell measurements. We tested the sensitivity and performance of a simple and rapid SARS-CoV-2...