Multiplexed staining of live human embryonic stem cells for flow cytometric analysis of pluripotency markers

• Published in: Stem cells and development Vol. 18; no. 8; p. 1135
• Main Authors: Prowse, Andrew B J, Wilson, John, Osborne, Geoffrey W, Gray, Peter P, Wolvetang, Ernst J
• Format: Journal Article
• Language: English
• Published: United States 01.10.2009
• Subjects: Biomarkers - analysis; Cell Differentiation; Cell Line; Cell Survival; Embryonic Stem Cells - chemistry; Embryonic Stem Cells - cytology; Flow Cytometry - methods; Humans; Pluripotent Stem Cells - chemistry; Pluripotent Stem Cells - cytology; Staining and Labeling - methods

Use of flow cytometry to detect pluripotency markers on or in human embryonic stem cells (hESCs) is a powerful analytical tool. However, current staining methodologies for high-content analysis of large numbers of samples utilize large quantities of primary and secondary antibodies, are time consumi...