Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics

• Published in: Journal of immunological methods Vol. 254; no. 1; pp. 109 - 118
• Main Authors: Cook, E.B., Stahl, J.L., Lowe, L., Chen, R., Morgan, E., Wilson, J., Varro, R., Chan, A., Graziano, F.M., Barney, N.P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.2001; Elsevier
• Subjects: Adult; Allergens - immunology; Allergy; Biological and medical sciences; Calibration; Cytokines; Cytokines - analysis; Cytometric bead array; Diverse techniques; Female; Flow cytometry; Flow Cytometry - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Male; Microbiology; Middle Aged; Molecular and cellular biology; Molecular immunology; Rhinitis, Allergic, Seasonal - immunology; Tears; Tears - immunology; Techniques; IFN, interferon; SAC, seasonal allergic conjunctivitis; Flow cytometry; Allergy; Cytokines; GM-CSF, granulocyte macrophage colony stimulating factor; TNF, tumor necrosis factor; Ab, antibody; FACS, fluorescence-activated cell sorter; Cytometric bead array; CBA, cytometric bead array; VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay; TH, T-helper; PE, phycoerythrin; TGF, transforming growth factor; IL, interleukin; VKC, vernal keratoconjunctivitis; MFI, mean fluorescence intensity; BDIS, Becton Dickinson Immunocytometry Systems; Tears; AKC, atopic keratoconjunctivitis; Human; Immunopathology; Multiple; Disease; Injury; Cytokine; Extracellular; Wound; Characterization; Particle; Regulation(control); Environment; Defense mechanism; Gamma interferon

Tears play an essential role in maintaining corneal and conjunctival integrity by providing a tightly regulated, optimal extracellular environment critical to its numerous functions, which include anti-microbial defense, wound healing and inflammatory responses such as allergies. Elevated levels of...