Chemiluminometric Immunosensor for High-Sensitivity Cardiac Troponin I Employing a Polymerized Enzyme Conjugate as a Tracer

• Published in: Scientific reports Vol. 5; no. 1; p. 14848
• Main Authors: Lim, Guei-Sam, Seo, Sung-Min, Paek, Sung-Ho, Kim, Seung-Wan, Jeon, Jin-Woo, Kim, Dong-Hyung, Cho, Il-Hoon, Paek, Se-Hwan
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 07.10.2015
• Subjects: Biosensing Techniques - methods; Biotin; Biotin - metabolism; Calcium-binding protein; Enzyme-Linked Immunosorbent Assay; Enzymes; Heart; Heart diseases; Horseradish peroxidase; Horseradish Peroxidase - metabolism; Humans; Immunoassay - methods; Limit of Detection; Luminescent Measurements - methods; Peroxidase; Streptavidin; Streptavidin - metabolism; Troponin; Troponin I; Troponin I - analysis; Troponin I - metabolism

To detect high-sensitivity cardiac troponin I (hs-cTnI; <0.01 ng/mL) at points of care, we developed a rapid immunosensor by using horseradish peroxidase polymerized in 20 molecules on average (Poly-HRP) as a tracer conjugated with streptavidin (SA-Poly-HRP). As shown in the conventional system,...