Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1

DOG1 (Delay of Germination 1) is a key regulator of seed dormancy in Arabidopsis (Arabidopsis thaliana) and other plants. Interestingly, the C terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis, DOG1 transcript is subject to alternative polyad...

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Published inPlant physiology (Bethesda) Vol. 170; no. 2; pp. 947 - 955
Main Authors Cyrek, Malgorzata, Fedak, Halina, Ciesielski, Arkadiusz, Guo, Yanwu, Sliwa, Aleksandra, Brzezniak, Lien, Krzyczmonik, Katarzyna, Pietras, Zbigniew, Kaczanowski, Szymon, Liu, Fuquan, Swiezewski, Szymon
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.02.2016
Subjects
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Base Sequence
Conserved Sequence
Gene Expression Regulation, Plant
GENES, DEVELOPMENT AND EVOLUTION
Germination
Molecular Sequence Data
Mutation - genetics
Phenotype
Plant Dormancy - genetics
Polyadenylation - genetics
Protein Biosynthesis
Protein Isoforms - genetics
Protein Isoforms - metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger - genetics
RNA, Messenger - metabolism
Seeds - genetics
Seeds - physiology
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Summary:DOG1 (Delay of Germination 1) is a key regulator of seed dormancy in Arabidopsis (Arabidopsis thaliana) and other plants. Interestingly, the C terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis, DOG1 transcript is subject to alternative polyadenylation. In line with this, mutants in RNA 3ʹ processing complex display weakened seed dormancy in parallel with defects in DOG1 proximal polyadenylation site selection, suggesting that the short DOG1 transcript is functional. This is corroborated by the finding that the proximally polyadenylated short DOG1 mRNA is translated in vivo and complements the dog1 mutant. In summary, our findings indicate that the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis and characterizes a set of mutants in RNA 3ʹ processing complex required for production of proximally polyadenylated functional DOG1 transcript.
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The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Szymon Swiezewski (sswiez@ibb.waw.pl).
Present address: Friedrich Miescher Institute, 4002 Basel, Switzerland.
www.plantphysiol.org/cgi/doi/10.1104/pp.15.01483
S.S. and F.L. designed the project and planned the program of research; H.F., M.C., and A.S. performed the germination tests and expression level measurements; H.F. and M.C. performed the western blots; L.B. did the RACE experiment; S.S. and S.K. performed the bioinformatics analysis; A.C. and H.F. examined localization; G.Y. performed the DOG1-GFP IP; L.B. and A.S. performed the RNA stability assay; K.K and Z.P. prepared the short and long DOG1 lines for complementation; S.S. and M.C. wrote the article.
This work was supported by MSHE (grant nos. IdP2011–000461 and 2011/01/D/NZ8/03690 to S.S.). H.F., M.C., A.S., and Z.P. were supported by Foundation for Polish Science Grant Number TEAM2010–5/9. L.B. was supported by IP2011 004671. S.S. was supported by EMBO Installation Grant CEBM/05/17.
These authors contributed equally to the article.
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.15.01483