Quantitative Viability Assessment of Microalgae for Advanced Wastewater Treatment by Flow Cytometry
The nutrient uptake capacity of microalgae can be applied in advanced wastewater treatment processes. This study was set to develop a rapid and effective method for assessing the viability (i.e., live or dead) of microalgae applied for advanced wastewater treatment using a flow cytometry. Especially...
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| Published in | KSCE journal of civil engineering Vol. 27; no. 9; pp. 3714 - 3719 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Seoul
Korean Society of Civil Engineers
01.09.2023
Springer Nature B.V 대한토목학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1226-7988 1976-3808 |
| DOI | 10.1007/s12205-023-0125-x |
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| Abstract | The nutrient uptake capacity of microalgae can be applied in advanced wastewater treatment processes. This study was set to develop a rapid and effective method for assessing the viability (i.e., live or dead) of microalgae applied for advanced wastewater treatment using a flow cytometry. Especially, a methodology to selectively evaluate the quantitative viability of only microalgae present in wastewaters having the co-culture of microalgae and bacteria was proposed. The live and dead cells of microalgae could be discriminated using chlorophyll autofluorescence and the SYTOX Green method developed in this study. Cell viability in live and dead conditions of microalgae showed about 90% accuracy, and about 40% viability ratio was derived in half-live and half-dead conditions. This result shows the potential to quantitatively evaluate the activity of microalgae in the wastewater environment. |
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| AbstractList | The nutrient uptake capacity of microalgae can be applied in advanced wastewater treatment processes. This study was set to develop a rapid and effective method for assessing the viability (i.e., live or dead) of microalgae applied for advanced wastewater treatment using a flow cytometry. Especially, a methodology to selectively evaluate the quantitative viability of only microalgae present in wastewaters having the co-culture of microalgae and bacteria was proposed. The live and dead cells of microalgae could be discriminated using chlorophyll autofluorescence and the SYTOX Green method developed in this study. Cell viability in live and dead conditions of microalgae showed about 90% accuracy, and about 40% viability ratio was derived in half-live and half-dead conditions. This result shows the potential to quantitatively evaluate the activity of microalgae in the wastewater environment. KCI Citation Count: 1 The nutrient uptake capacity of microalgae can be applied in advanced wastewater treatment processes. This study was set to develop a rapid and effective method for assessing the viability (i.e., live or dead) of microalgae applied for advanced wastewater treatment using a flow cytometry. Especially, a methodology to selectively evaluate the quantitative viability of only microalgae present in wastewaters having the co-culture of microalgae and bacteria was proposed. The live and dead cells of microalgae could be discriminated using chlorophyll autofluorescence and the SYTOX Green method developed in this study. Cell viability in live and dead conditions of microalgae showed about 90% accuracy, and about 40% viability ratio was derived in half-live and half-dead conditions. This result shows the potential to quantitatively evaluate the activity of microalgae in the wastewater environment. |
| Author | Jho, Eun Hea Hwang, Sun-Jin Park, Kyu-Hyun |
| Author_xml | – sequence: 1 givenname: Kyu-Hyun surname: Park fullname: Park, Kyu-Hyun organization: Dept. of Environmental Science and Engineering, Kyung Hee University – sequence: 2 givenname: Eun Hea surname: Jho fullname: Jho, Eun Hea organization: Dept. of Agricultural and Biological Chemistry, Chonnam National University – sequence: 3 givenname: Sun-Jin surname: Hwang fullname: Hwang, Sun-Jin email: sjhwang@khu.ac.kr organization: Dept. of Environmental Science and Engineering, Kyung Hee University |
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| CitedBy_id | crossref_primary_10_1016_j_cej_2024_156424 crossref_primary_10_1016_j_algal_2024_103435 crossref_primary_10_3390_membranes15010035 |
| Cites_doi | 10.1016/0734-9750(88)91921-0 10.1038/s41598-017-05027-3 10.1016/j.scitotenv.2020.142168 10.1007/BF01065839 10.1016/j.algal.2020.101943 10.3989/scimar.2000.64n2197 10.1007/s10811-020-02304-z 10.1093/plankt/fbs018 10.1002/1097-0320(20010701)44:3<236::AID-CYTO1116>3.0.CO;2-5 10.1016/j.biortech.2016.09.123 10.1016/j.scitotenv.2022.155110 10.1007/s10750-014-1952-z 10.1002/cyto.a.23851 10.1111/j.1529-8817.2011.00991.x 10.1016/j.algal.2019.101666 10.2166/wst.2009.547 10.1016/j.enzmictec.2003.01.001 10.1016/j.biotechadv.2012.04.007 10.1016/j.biortech.2017.08.135 10.2166/wst.1993.0504 10.1007/s12205-017-0730-7 10.1016/j.biortech.2008.08.019 10.1111/j.1751-1097.1982.tb09484.x |
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| Keywords | Flow cytometry Advanced wastewater treatment Cell viability Microalgae Chlorophyll autofluorescence |
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| SubjectTerms | Advanced wastewater treatment Algae Aquatic microorganisms Bacteria Carbon Cell culture Cell viability Cells Chlorophyll Chlorophylls Civil Engineering Engineering Environmental Engineering Environmental science Flow cytometry Geotechnical Engineering & Applied Earth Sciences Industrial Pollution Prevention Metabolism Microalgae Nitrogen Nutrient uptake Photosynthesis Phytoplankton Uptake Wastewater treatment Water treatment 토목공학 |
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