Immaturity of human stem-cell-derived cardiomyocytes in culture: fatal flaw or soluble problem?
Cardiomyocytes from human pluripotent stem cells (hPSC-CMs) are increasingly used to model cardiac disease, test drug efficacy and for safety pharmacology. Nevertheless, a major hurdle to more extensive use is their immaturity and similarity to fetal rather than adult cardiomyocytes. Here, we provid...
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| Published in | Stem cells and development Vol. 24; no. 9; p. 1035 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
01.05.2015
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| Subjects | |
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| Abstract | Cardiomyocytes from human pluripotent stem cells (hPSC-CMs) are increasingly used to model cardiac disease, test drug efficacy and for safety pharmacology. Nevertheless, a major hurdle to more extensive use is their immaturity and similarity to fetal rather than adult cardiomyocytes. Here, we provide an overview of the strategies currently being used to increase maturation in culture, which include prolongation of time in culture, exposure to electrical stimulation, application of mechanical strain, growth in three-dimensional tissue configuration, addition of non-cardiomyocytes, use of hormones and small molecules, and alteration of the extracellular environment. By comparing the outcomes of these studies, we identify the approaches most likely to improve functional maturation of hPSC-CMs in terms of their electrophysiology and excitation-contraction coupling. |
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| AbstractList | Cardiomyocytes from human pluripotent stem cells (hPSC-CMs) are increasingly used to model cardiac disease, test drug efficacy and for safety pharmacology. Nevertheless, a major hurdle to more extensive use is their immaturity and similarity to fetal rather than adult cardiomyocytes. Here, we provide an overview of the strategies currently being used to increase maturation in culture, which include prolongation of time in culture, exposure to electrical stimulation, application of mechanical strain, growth in three-dimensional tissue configuration, addition of non-cardiomyocytes, use of hormones and small molecules, and alteration of the extracellular environment. By comparing the outcomes of these studies, we identify the approaches most likely to improve functional maturation of hPSC-CMs in terms of their electrophysiology and excitation-contraction coupling. |
| Author | Verkerk, Arie O Casini, Simona Bellin, Milena Kosmidis, Georgios Mummery, Christine L Veerman, Christiaan C |
| Author_xml | – sequence: 1 givenname: Christiaan C surname: Veerman fullname: Veerman, Christiaan C organization: 1 Department of Experimental Cardiology, Academic Medical Center, University of Amsterdam , Amsterdam, the Netherlands – sequence: 2 givenname: Georgios surname: Kosmidis fullname: Kosmidis, Georgios – sequence: 3 givenname: Christine L surname: Mummery fullname: Mummery, Christine L – sequence: 4 givenname: Simona surname: Casini fullname: Casini, Simona – sequence: 5 givenname: Arie O surname: Verkerk fullname: Verkerk, Arie O – sequence: 6 givenname: Milena surname: Bellin fullname: Bellin, Milena |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25583389$$D View this record in MEDLINE/PubMed |
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| Title | Immaturity of human stem-cell-derived cardiomyocytes in culture: fatal flaw or soluble problem? |
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