Multicolor sparse viral labeling and 3D digital tracing of enteric plexus in mouse proximal colon using a novel adeno‐associated virus capsid

• Published in: Neurogastroenterology and motility Vol. 33; no. 8; pp. e14014 - n/a
• Main Authors: Wang, Lixin, Challis, Collin, Li, Songlin, Fowlkes, Charless C., Ravindra Kumar, Sripriya, Yuan, Pu‐Qing, Taché, Yvette F.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2021
• Subjects: 3D imaging; adeno‐associated virus; Animals; Apposition; Axons; Colon; Colon - innervation; Colon - metabolism; colonic enteric neuron; Dependovirus; digital tracing; Enteric nervous system; Enteric Nervous System - metabolism; Enteric Nervous System - virology; Female; Fibers; Fluorophores; Gene Transfer Techniques; Green Fluorescent Proteins; Intravenous administration; Labeling; Male; Mice; mouse; Myenteric plexus; Nerve endings; Nervous system; Neurons; Regulatory sequences; Submucosal plexus; Submucous Plexus - metabolism; Submucous Plexus - virology; digital tracing; mouse; adeno-associated virus; 3D imaging; colonic enteric neuron

Background Intravenous administration of adeno‐associated virus (AAV) can be used as a noninvasive approach to trace neuronal morphology and links. AAV‐PHP.S is a variant of AAV9 that effectively transduces the peripheral nervous system. The objective was to label randomly and sparsely enteric plexu...