Photodamage attenuating potential of Nectandra hihua against UVB-induced oxidative stress in L929 fibroblasts

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 181; pp. 127 - 133
• Main Authors: Oliveira, Mariana Maciel de, Daré, Regina Gomes, Barizão, Érica Oliveira, Visentainer, Jesuí Vergílio, Romagnolo, Mariza Barion, Nakamura, Celso Vataru, Truiti, Maria da Conceição Torrado
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01.04.2018; Elsevier BV
• Subjects: Acetic acid; Antioxidants; Butylated hydroxytoluene; Damage prevention; Ethyl acetate; Fibroblasts; Flavonoids; Inhibition; L929 fibroblasts; Leaves; Lipid peroxidation; Nectandra hihua; Oxidative stress; Peroxidation; Phenolic compounds; Phenols; Photochemoprotective effect; Pretreatment; Quercetin; Reactive oxygen species; Skin; Ultraviolet radiation; UVB radiation; Oxidative stress; L929 fibroblasts; UVB radiation; Photochemoprotective effect
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2018.03.008

The total phenolic content (TP) and antioxidant activity of the ethanolic extract and fractions that were obtained from the leaves of Nectandra hihua were assessed using different methods. The ethanolic extract (EE) and ethyl acetate fraction (EAF) had the best antioxidant capacity, which was comparable to butylated hydroxytoluene and quercetin (ABTS+ 2.55 ± 0.06, 3.54 ± 0.03 mmol TE/g; DPPH IC50 10.27 ± 0.05, 9.88 ± 0.02 μg/mL; FRAP 2.17 ± 0.08, 2.38 ± 0.04 mmol TE/g; ORAC 5.16 ± 0.08, 5.35 ± 0.07 mmol TE/g; TP 568.05 ± 18.15, 397.20 ± 17.88 mg GAE/g, respectively). The cytoprotective effect, reactive oxygen species (ROS) and lipid peroxidation inhibitions on L929 fibroblasts irradiated with UVB (600 mJ/cm2) in pre- and post-treatments with EE and EAF were determined. These plant materials demonstrated high ROS scavenging activity and lipid peroxidation inhibition on L929 fibroblasts in both treatments, especially with pre-treatment (EE 38.47 ± 1.95% and EAF 40.20 ± 4.5% inhibition of ROS production, and EE 39.03 ± 3.33% and EAF 41.67 ± 7.6% of lipid peroxidation inhibition), indicating the best cytoprotection with pre-treatment (13.52 ± 1.66% and 13.34 ± 2.61% increases in cell viability). The antioxidant flavonoids quercitrin, avicularin, juglalin, afzelin and astragalin were isolated from EAF. The results obtained indicate that EE and EAF present photodamage attenuating potential against UVB-induced oxidative stress and can be useful as a starting point for developing dermatological products to prevent oxidative skin damage. •Photodamage attenuating potential of Nectandra hihua was assessed.•Plant materials of N. hihua presented powerful in vitro antioxidant capacity.•Plant materials were capable to prevent, and attenuate cellular damage UVB-induced.•Quercitrin, avicularin, juglalin, afzelin and astragalin were identified.