Flow injection method for the determination of thyroxine by inhibition of glutamate dehydrogenase

• Published in: Analytica chimica acta Vol. 411; no. 1; pp. 45 - 49
• Main Authors: Ghous, T, Townshend, A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2000; Elsevier
• Subjects: Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Enzyme inhibition; Flow injection; Fundamental and applied biological sciences. Psychology; Glutamate dehydrogenase; Proteins; Spectrophotometry; Thyroxine; Glutamate dehydrogenase; Enzyme inhibition; Spectrophotometry; Thyroxine; Flow injection; Analysis method; Enzyme; Aminoacid derivative hormone; Thyroid hormone; Oxidoreductases; Inhibition; Quantitative analysis

Thyroxine is an important hormone and is reported to be an inhibitor of glutamate dehydrogenase (GlDH). This inhibitory effect of thyroxine is used to develop a simple and highly reproducible flow injection method for its determination. The change in NADH absorbance at 340 nm in the presence of the...