Frequency-shift vs phase-shift characterization of in-liquid quartz crystal microbalance applications

• Published in: Review of scientific instruments Vol. 82; no. 6; p. 064702
• Main Authors: Montagut, Y J, García, J V, Jiménez, Y, March, C, Montoya, A, Arnau, A
• Format: Journal Article
• Language: English
• Published: United States 01.06.2011
• Subjects: Biosensing Techniques; Calibration; Electricity; Immunoassay; Quartz Crystal Microbalance Techniques - instrumentation; Reproducibility of Results
• ISSN: 1089-7623
• DOI: 10.1063/1.3598340

The improvement of sensitivity in quartz crystal microbalance (QCM) applications has been addressed in the last decades by increasing the sensor fundamental frequency, following the increment of the frequency/mass sensitivity with the square of frequency predicted by Sauerbrey. However, this sensitivity improvement has not been completely transferred in terms of resolution. The decrease of frequency stability due to the increase of the phase noise, particularly in oscillators, made impossible to reach the expected resolution. A new concept of sensor characterization at constant frequency has been recently proposed. The validation of the new concept is presented in this work. An immunosensor application for the detection of a low molecular weight contaminant, the insecticide carbaryl, has been chosen for the validation. An, in principle, improved version of a balanced-bridge oscillator is validated for its use in liquids, and applied for the frequency shift characterization of the QCM immunosensor application. The classical frequency shift characterization is compared with the new phase-shift characterization concept and system proposed.