Identification of the key amino acid sites of the carbendazim hydrolase (MheI) from a novel carbendazim-degrading strain Mycobacterium sp. SD-4
•Strain SD-4 was the first MBC-degrading strain isolated from the genus of Mycobacterium.•The codon of mheI was optimized to realize its soluble expression in E.coil.•Key amino acid sites of MheI were identified. A novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC) degrading strain SD-...
Saved in:
| Published in | Journal of hazardous materials Vol. 331; pp. 55 - 62 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
05.06.2017
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | •Strain SD-4 was the first MBC-degrading strain isolated from the genus of Mycobacterium.•The codon of mheI was optimized to realize its soluble expression in E.coil.•Key amino acid sites of MheI were identified.
A novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC) degrading strain SD-4 was isolated and identified preliminarily as Mycobacterium sp. according to its phenotypic features and phylogenetic analysis. This strain could utilize MBC as the sole carbon and nitrogen sources for growth and degrade 50mgL−1 MBC at the average degradation rate of 0.63mgL−1h−1. Strain SD-4 degraded MBC through the typical pathway, in which MBC was first hydrolyzed by MheI to 2-aminobenzimidazole (2-AB) and then converted to 2-hydroxybenzimidazole (2-HB). The MBC hydrolase encoding gene mheI was cloned from strain SD-4 and successfully expressed in Escherichia coli by codon optimization. The sulfhydryl-blocking assay revealed that the activity of MheI was closely related to cysteine, and the site-directed mutation experiment showed that Cys16 and Cys222 played important roles during the hydrolysis of MBC by MheI. Therefore they affected its activity directly and were defined as the key amino acid sites. |
|---|---|
| AbstractList | A novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC) degrading strain SD-4 was isolated and identified preliminarily as Mycobacterium sp. according to its phenotypic features and phylogenetic analysis. This strain could utilize MBC as the sole carbon and nitrogen sources for growth and degrade 50mgL
MBC at the average degradation rate of 0.63mgL
h
. Strain SD-4 degraded MBC through the typical pathway, in which MBC was first hydrolyzed by MheI to 2-aminobenzimidazole (2-AB) and then converted to 2-hydroxybenzimidazole (2-HB). The MBC hydrolase encoding gene mheI was cloned from strain SD-4 and successfully expressed in Escherichia coli by codon optimization. The sulfhydryl-blocking assay revealed that the activity of MheI was closely related to cysteine, and the site-directed mutation experiment showed that Cys16 and Cys222 played important roles during the hydrolysis of MBC by MheI. Therefore they affected its activity directly and were defined as the key amino acid sites. •Strain SD-4 was the first MBC-degrading strain isolated from the genus of Mycobacterium.•The codon of mheI was optimized to realize its soluble expression in E.coil.•Key amino acid sites of MheI were identified. A novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC) degrading strain SD-4 was isolated and identified preliminarily as Mycobacterium sp. according to its phenotypic features and phylogenetic analysis. This strain could utilize MBC as the sole carbon and nitrogen sources for growth and degrade 50mgL−1 MBC at the average degradation rate of 0.63mgL−1h−1. Strain SD-4 degraded MBC through the typical pathway, in which MBC was first hydrolyzed by MheI to 2-aminobenzimidazole (2-AB) and then converted to 2-hydroxybenzimidazole (2-HB). The MBC hydrolase encoding gene mheI was cloned from strain SD-4 and successfully expressed in Escherichia coli by codon optimization. The sulfhydryl-blocking assay revealed that the activity of MheI was closely related to cysteine, and the site-directed mutation experiment showed that Cys16 and Cys222 played important roles during the hydrolysis of MBC by MheI. Therefore they affected its activity directly and were defined as the key amino acid sites. A novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC) degrading strain SD-4 was isolated and identified preliminarily as Mycobacterium sp. according to its phenotypic features and phylogenetic analysis. This strain could utilize MBC as the sole carbon and nitrogen sources for growth and degrade 50mgL-1 MBC at the average degradation rate of 0.63mgL-1h-1. Strain SD-4 degraded MBC through the typical pathway, in which MBC was first hydrolyzed by MheI to 2-aminobenzimidazole (2-AB) and then converted to 2-hydroxybenzimidazole (2-HB). The MBC hydrolase encoding gene mheI was cloned from strain SD-4 and successfully expressed in Escherichia coli by codon optimization. The sulfhydryl-blocking assay revealed that the activity of MheI was closely related to cysteine, and the site-directed mutation experiment showed that Cys16 and Cys222 played important roles during the hydrolysis of MBC by MheI. Therefore they affected its activity directly and were defined as the key amino acid sites. |
| Author | Hong, Qing Wang, Xiang Zhu, Shijun Hu, Gang Wang, Hui Zhang, Chenfei Zhang, Yingkun Jin, Wen Hu, Bo |
| Author_xml | – sequence: 1 givenname: Yingkun surname: Zhang fullname: Zhang, Yingkun organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 2 givenname: Hui surname: Wang fullname: Wang, Hui organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 3 givenname: Xiang surname: Wang fullname: Wang, Xiang organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 4 givenname: Bo surname: Hu fullname: Hu, Bo organization: Industrial Product Division, Intrexon Corporation, South San Francisco, CA, 94080, USA – sequence: 5 givenname: Chenfei surname: Zhang fullname: Zhang, Chenfei organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 6 givenname: Wen surname: Jin fullname: Jin, Wen organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 7 givenname: Shijun surname: Zhu fullname: Zhu, Shijun organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 8 givenname: Gang surname: Hu fullname: Hu, Gang organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China – sequence: 9 givenname: Qing surname: Hong fullname: Hong, Qing email: hongqing@njau.edu.cn organization: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of life sciences, Nanjing Agricultural University, Nanjing, 210095, PR China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28242529$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkc1O3DAUha2KqgzQR2jlJV0k-C9xsqoqaMtIoC5gbzn2DeNpYk9tD9LwEn3lGs1QsWN1F_c79-iec4KOfPCA0CdKakpoe7Gu1yv9NOtcM0JlTVhNiHyHFrSTvOKct0doQTgRFe96cYxOUloTUshGfEDHrGOCNaxfoL9LCz670RmdXfA4jDivAP-GHdaz8wFr4yxOLkN62RkdB_BWP7kZr3Y2hkknwOe3K1h-wWMMM9bYh0eYXpOVhYeorfMPOOWonce3OxMGbTJEt51x2tT47qoSZ-j9qKcEHw_zFN3_-H5_eV3d_Pq5vPx2UxlBWK5kR0z5YTC2sQNvLB8IF00HIxNaGqMZN5Z1fWNa29MeCjvQvmm1aHspe8pP0fn-7CaGP1tIWc0uGZgm7SFskyopsk4S2YqCNnvUxJBShFFtopt13ClK1HMVaq0OVajnKhRhqlRRdJ8PFtthBvtf9ZJ9Ab7uASh_PjqIKhkH3oB1EUxWNrg3LP4BBxmfyQ |
| CitedBy_id | crossref_primary_10_1080_03067319_2022_2053847 crossref_primary_10_1080_10889868_2021_1911921 crossref_primary_10_1016_j_jhazmat_2020_125026 crossref_primary_10_1093_femsle_fnad085 crossref_primary_10_1016_j_jhazmat_2017_12_006 crossref_primary_10_1002_etc_5534 crossref_primary_10_1016_j_chemosphere_2022_137723 crossref_primary_10_1016_j_envres_2024_119290 crossref_primary_10_1016_j_jhazmat_2019_04_089 crossref_primary_10_1016_j_jece_2021_107024 crossref_primary_10_1186_s12934_021_01698_w crossref_primary_10_1021_acs_jafc_8b06179 crossref_primary_10_1007_s42729_023_01254_y crossref_primary_10_1016_j_envpol_2018_09_080 crossref_primary_10_1016_j_jhazmat_2023_132847 crossref_primary_10_3389_fmicb_2021_648868 crossref_primary_10_1016_j_apsoil_2019_09_006 crossref_primary_10_1016_j_jhazmat_2021_125496 crossref_primary_10_3390_molecules28020719 crossref_primary_10_1016_j_micres_2022_127081 crossref_primary_10_3389_fmicb_2022_842736 crossref_primary_10_1016_j_jhazmat_2022_130293 crossref_primary_10_1021_acs_jafc_8b02449 crossref_primary_10_1016_j_scitotenv_2019_135726 crossref_primary_10_1021_acs_est_9b01037 crossref_primary_10_1016_j_micres_2023_127564 crossref_primary_10_1016_j_scitotenv_2020_142137 crossref_primary_10_1016_j_jhazmat_2023_132379 crossref_primary_10_1016_j_chemosphere_2022_135231 crossref_primary_10_1111_1462_2920_16139 crossref_primary_10_3390_microorganisms9030502 |
| Cites_doi | 10.1016/j.molbiopara.2016.06.009 10.1016/j.carres.2016.08.009 10.1080/19443994.2013.879081 10.1016/S1016-8478(23)13989-6 10.1007/s00284-005-0474-3 10.1007/s10311-002-0010-7 10.1128/mBio.02161-15 10.1016/j.chemosphere.2010.08.040 10.1016/S0278-6915(97)00116-6 10.1007/BF01977627 10.1002/bmc.1130090104 10.1016/0165-1218(94)90119-8 10.1016/j.etap.2008.11.006 10.1007/s10661-014-4027-8 10.1016/j.sbi.2016.07.004 10.1099/ijs.0.038075-0 10.1016/j.bbagen.2009.01.014 10.1093/molbev/mst197 10.1016/j.jmb.2010.09.027 10.1016/0003-2697(76)90527-3 10.1021/jf00002a035 10.1016/j.reprotox.2007.03.010 10.1016/S0887-2333(03)00103-6 10.1016/j.jhazmat.2007.06.039 10.1080/00498250802651976 10.1021/bi9628580 10.1159/000225079 10.1093/nar/25.24.4876 10.1016/S0040-8166(02)00006-X 10.1128/AEM.02990-09 10.1016/S0045-6535(02)00620-3 10.1016/j.jmb.2009.09.052 10.1016/j.jhazmat.2009.06.142 10.1021/cb3005269 10.1016/S0166-445X(99)00037-5 10.1007/s10532-010-9353-0 |
| ContentType | Journal Article |
| Copyright | 2017 Elsevier B.V. Copyright © 2017 Elsevier B.V. All rights reserved. |
| Copyright_xml | – notice: 2017 Elsevier B.V. – notice: Copyright © 2017 Elsevier B.V. All rights reserved. |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
| DOI | 10.1016/j.jhazmat.2017.02.007 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering Law |
| EISSN | 1873-3336 |
| EndPage | 62 |
| ExternalDocumentID | 10_1016_j_jhazmat_2017_02_007 28242529 S0304389417300882 |
| Genre | Journal Article |
| GroupedDBID | --- --K --M -~X ..I .DC .~1 0R~ 1B1 1RT 1~. 1~5 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM 9JN AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAXUO ABFNM ABFRF ABFYP ABJNI ABLST ABMAC ABNUV ABYKQ ACDAQ ACGFO ACGFS ACRLP ADBBV ADEWK ADEZE AEBSH AEFWE AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGUBO AGYEJ AHEUO AHHHB AHPOS AIEXJ AIKHN AITUG AJBFU AJOXV AKIFW AKURH ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLECG BLXMC CS3 DU5 EBS EFJIC EFLBG EJD ENUVR EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FYGXN G-Q GBLVA IHE J1W KCYFY KOM LX7 LY9 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 RIG ROL RPZ SDF SDG SDP SES SPC SPCBC SSG SSJ SSZ T5K XPP ZMT ~02 ~G- AAHBH AAXKI AKRWK CGR CUY CVF ECM EIF NPM .HR 29K AAQXK AAYXX ABXDB ADMUD AFJKZ AI. ASPBG AVWKF AZFZN BBWZM CITATION D-I F3I FEDTE FGOYB G-2 HLY HMC HVGLF HZ~ NDZJH R2- SCE SEN SEW T9H TAE UAO VH1 WUQ 7X8 |
| ID | FETCH-LOGICAL-c402t-780c824bcd5db35d3b03458ef24a7cca23cd2895c6d919ec82b1956a46977913 |
| IEDL.DBID | AIKHN |
| ISSN | 0304-3894 |
| IngestDate | Fri Aug 16 21:00:39 EDT 2024 Thu Sep 26 21:38:53 EDT 2024 Wed Oct 16 00:57:16 EDT 2024 Fri Feb 23 02:29:19 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | mheI Mycobacterium sp. SD-4 Expression Carbendazim Key amino acid sites |
| Language | English |
| License | Copyright © 2017 Elsevier B.V. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c402t-780c824bcd5db35d3b03458ef24a7cca23cd2895c6d919ec82b1956a46977913 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 28242529 |
| PQID | 1872870764 |
| PQPubID | 23479 |
| PageCount | 8 |
| ParticipantIDs | proquest_miscellaneous_1872870764 crossref_primary_10_1016_j_jhazmat_2017_02_007 pubmed_primary_28242529 elsevier_sciencedirect_doi_10_1016_j_jhazmat_2017_02_007 |
| PublicationCentury | 2000 |
| PublicationDate | 2017-06-05 |
| PublicationDateYYYYMMDD | 2017-06-05 |
| PublicationDate_xml | – month: 06 year: 2017 text: 2017-06-05 day: 05 |
| PublicationDecade | 2010 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Journal of hazardous materials |
| PublicationTitleAlternate | J Hazard Mater |
| PublicationYear | 2017 |
| Publisher | Elsevier B.V |
| Publisher_xml | – name: Elsevier B.V |
| References | Mazellier, Leroy, De Laat, Legube (bib0005) 2003; 1 Kiigemagi, Inman, Mellenthin, Deinzer (bib0015) 1991; 39 Parret, Besir, Meijers (bib0150) 2016; 38 Singhal, Bagga, Kumar, Chauhan (bib0035) 2003; 17 Arner (bib0195) 2009; 1790 Nakai, Miller, Carnes, Hess (bib0040) 2002; 34 Xu, Gu, Biao, Wang, Kun, Li (bib0100) 2006; 53 Kim, Cho, Lee, Yoon, Kim, Na, Park, Jeon, Lee, Yi, Won, Chun (bib0110) 2012; 62 Ramakrishna, Venkata Mohan, Shailaja, Narashima, Sarma (bib0140) 2008; 151 Holt, Krieg, Sneath, Staley, Williams (bib0105) 1994 Chivers, Prehoda, Raines (bib0180) 1997; 36 Wang, Huang, Chen, Yen (bib0055) 2009; 172 Thompson, Gibson, Plewniak, Jeanmougin, Higgins (bib0120) 1997; 25 Imberti, Mapelli, Colombo, Richelmi, Berte, Bellomo (bib0190) 1990; 64 Mantovani, Maranghi, Ricciardi, Macri, Stazi, Attias, Zapponi (bib0060) 1998; 36 Sarrif, Arce, Krahn, O'Neil, Reynolds (bib0030) 1994; 321 Yu, Liu, Xie, Wang (bib0045) 2009; 27 Kaur, Toor, Wanchoo (bib0065) 2015; 54 Wei, Xu, Guo, Shi, Sun (bib0070) 2015; 43 Boudina, Emmelin, Baahiouamer, Grenier-Loustalot, Chovelon (bib0010) 2003; 50 Fomenko, Marino, Gladyshev (bib0175) 2008; 26 Wang, Wang, Gong, Zhang, Hong, Li (bib0095) 2010; 81 Bradford (bib0130) 1976; 72 Pace, Weerapana (bib0155) 2013; 8 Jeong, Barbe, Lee, Vallenet, Yu, Choi, Couloux, Lee, Yoon, Cattolico, Hur, Park, Segurens, Kim, Oh, Lenski, Studier, Daegelen, Kim (bib0145) 2009; 394 Pandey, Dorrian, Russell, Brearley, Kotsonis, Oakeshott (bib0080) 2010; 76 Sun, Horikawa, Wada, Sugiyama, Imai (bib0200) 2016; 434 Marino, Gladyshev (bib0170) 2010; 404 Salunkhe, Sawant, Banerjee, Wadkar, Sawant, Hingmire (bib0075) 2014; 186 Fang, Wang, Gao, Yan, Dong, Yu (bib0090) 2010; 21 Chandra, Datta (bib0160) 2016; 208 Van den Brink, Hattink, Bransen, Van Donk, Brock (bib0025) 2000; 48 Tamura, Stecher, Peterson, Filipski, Kumar (bib0115) 2013; 30 Joshi, Heasley, Macdonald (bib0185) 2009; 39 Zhang, Huang, Harvey, Li, Ren, Li, Wang, Yang (bib0085) 2013; 8 Saitou, Nei (bib0125) 1987; 4 Knock, Stein, Davis, Galt, Oester, Sylvester (bib0135) 1975; 32 Thapar, Bhushan, Mathur (bib0020) 1995; 9 Crawford, Tapscott, Fitzsimmons, Liu, Reyes, Libby, Trujillo, Fang, Radi, Vazquez-Torres (bib0165) 2016; 7 Rajeswary, Kumarana, Ilangovan, Yuvaraj, Sridhar, Venkataraman, Srinivasan, Aruldhas (bib0050) 2007; 24 Thapar (10.1016/j.jhazmat.2017.02.007_bib0020) 1995; 9 Chandra (10.1016/j.jhazmat.2017.02.007_bib0160) 2016; 208 Xu (10.1016/j.jhazmat.2017.02.007_bib0100) 2006; 53 Knock (10.1016/j.jhazmat.2017.02.007_bib0135) 1975; 32 Wei (10.1016/j.jhazmat.2017.02.007_bib0070) 2015; 43 Yu (10.1016/j.jhazmat.2017.02.007_bib0045) 2009; 27 Wang (10.1016/j.jhazmat.2017.02.007_bib0095) 2010; 81 Pandey (10.1016/j.jhazmat.2017.02.007_bib0080) 2010; 76 Zhang (10.1016/j.jhazmat.2017.02.007_bib0085) 2013; 8 Pace (10.1016/j.jhazmat.2017.02.007_bib0155) 2013; 8 Mazellier (10.1016/j.jhazmat.2017.02.007_bib0005) 2003; 1 Chivers (10.1016/j.jhazmat.2017.02.007_bib0180) 1997; 36 Arner (10.1016/j.jhazmat.2017.02.007_bib0195) 2009; 1790 Sarrif (10.1016/j.jhazmat.2017.02.007_bib0030) 1994; 321 Fang (10.1016/j.jhazmat.2017.02.007_bib0090) 2010; 21 Sun (10.1016/j.jhazmat.2017.02.007_bib0200) 2016; 434 Singhal (10.1016/j.jhazmat.2017.02.007_bib0035) 2003; 17 Mantovani (10.1016/j.jhazmat.2017.02.007_bib0060) 1998; 36 Wang (10.1016/j.jhazmat.2017.02.007_bib0055) 2009; 172 Boudina (10.1016/j.jhazmat.2017.02.007_bib0010) 2003; 50 Rajeswary (10.1016/j.jhazmat.2017.02.007_bib0050) 2007; 24 Joshi (10.1016/j.jhazmat.2017.02.007_bib0185) 2009; 39 Kim (10.1016/j.jhazmat.2017.02.007_bib0110) 2012; 62 Ramakrishna (10.1016/j.jhazmat.2017.02.007_bib0140) 2008; 151 Marino (10.1016/j.jhazmat.2017.02.007_bib0170) 2010; 404 Thompson (10.1016/j.jhazmat.2017.02.007_bib0120) 1997; 25 Fomenko (10.1016/j.jhazmat.2017.02.007_bib0175) 2008; 26 Jeong (10.1016/j.jhazmat.2017.02.007_bib0145) 2009; 394 Van den Brink (10.1016/j.jhazmat.2017.02.007_bib0025) 2000; 48 Salunkhe (10.1016/j.jhazmat.2017.02.007_bib0075) 2014; 186 Holt (10.1016/j.jhazmat.2017.02.007_bib0105) 1994 Bradford (10.1016/j.jhazmat.2017.02.007_bib0130) 1976; 72 Parret (10.1016/j.jhazmat.2017.02.007_bib0150) 2016; 38 Tamura (10.1016/j.jhazmat.2017.02.007_bib0115) 2013; 30 Saitou (10.1016/j.jhazmat.2017.02.007_bib0125) 1987; 4 Crawford (10.1016/j.jhazmat.2017.02.007_bib0165) 2016; 7 Nakai (10.1016/j.jhazmat.2017.02.007_bib0040) 2002; 34 Imberti (10.1016/j.jhazmat.2017.02.007_bib0190) 1990; 64 Kiigemagi (10.1016/j.jhazmat.2017.02.007_bib0015) 1991; 39 Kaur (10.1016/j.jhazmat.2017.02.007_bib0065) 2015; 54 |
| References_xml | – volume: 1 start-page: 68 year: 2003 end-page: 72 ident: bib0005 article-title: Degradation of carbendazim by UV/H2O2 investigated by kinetic modelling publication-title: Environ. Chem. Lett. contributor: fullname: Legube – volume: 38 start-page: 155 year: 2016 end-page: 162 ident: bib0150 article-title: Critical reflections on synthetic gene design for recombinant protein expression publication-title: Curr. Opin. Struct. Biol. contributor: fullname: Meijers – volume: 404 start-page: 902 year: 2010 end-page: 916 ident: bib0170 article-title: Cysteine function governs its conservation and degeneration and restricts its utilization on protein surfaces publication-title: J. Mol. Biol. contributor: fullname: Gladyshev – volume: 7 year: 2016 ident: bib0165 article-title: Redox-active sensing by bacterial DksA transcription factors is determined by cysteine and zinc content publication-title: Mbio contributor: fullname: Vazquez-Torres – volume: 48 start-page: 251 year: 2000 end-page: 264 ident: bib0025 article-title: Impact of the fungicide carbendazim in freshwater microcosms. II. Zooplankton, primary producers and final conclusions publication-title: Aquat. Toxicol. contributor: fullname: Brock – volume: 27 start-page: 287 year: 2009 end-page: 292 ident: bib0045 article-title: Involvement of Sertoli cells in spermatogenic failure induced by carbendazim publication-title: Environ. Toxicol. Pharm. contributor: fullname: Wang – volume: 172 start-page: 84 year: 2009 end-page: 91 ident: bib0055 article-title: Effect of carbendazim and pencycuron on soil bacterial community publication-title: J. Hazard. Mater. contributor: fullname: Yen – volume: 30 start-page: 2725 year: 2013 end-page: 2729 ident: bib0115 article-title: MEGA6: molecular evolutionary genetics analysis version 6.0 publication-title: Mol. Biol. Evolut. contributor: fullname: Kumar – year: 1994 ident: bib0105 article-title: Bergey’s Manual of Determinate Bacteriology contributor: fullname: Williams – volume: 50 start-page: 649 year: 2003 end-page: 655 ident: bib0010 article-title: Photochemical behaviour of carbendazim in aqueous solution publication-title: Chemosphere contributor: fullname: Chovelon – volume: 81 start-page: 639 year: 2010 end-page: 644 ident: bib0095 article-title: Biodegradation of carbendazim by a novel actinobacterium Rhodococcus jialingiae djl-6-2 publication-title: Chemosphere contributor: fullname: Li – volume: 62 start-page: 716 year: 2012 end-page: 721 ident: bib0110 article-title: Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species publication-title: Int. J. Syst. Evolut. Microbiol. contributor: fullname: Chun – volume: 8 start-page: 283 year: 2013 end-page: 296 ident: bib0155 article-title: Diverse functional roles of reactive cysteines publication-title: ACS Chem. Biol. contributor: fullname: Weerapana – volume: 186 start-page: 8599 year: 2014 end-page: 8610 ident: bib0075 article-title: Kinetics of degradation of carbendazim by B-subtilis strains: possibility of in situ detoxification publication-title: Environ. Monit. Assess. contributor: fullname: Hingmire – volume: 17 start-page: 687 year: 2003 end-page: 692 ident: bib0035 article-title: Down regulation of humoral immunity in chickens due to carbendazim publication-title: Toxicol. in Vitro contributor: fullname: Chauhan – volume: 32 start-page: 291 year: 1975 end-page: 301 ident: bib0135 article-title: Effects of selected sulfhydryl inhibitors on nonhistone chromosomal proteins of HeLa cells publication-title: Oncology contributor: fullname: Sylvester – volume: 208 start-page: 84 year: 2016 end-page: 90 ident: bib0160 article-title: Role of cysteine residues in the redox-regulated oligomerization and nucleotide binding to EhRabX3 publication-title: Mol. Biochem. Parasitol. contributor: fullname: Datta – volume: 1790 start-page: 495 year: 2009 end-page: 526 ident: bib0195 article-title: Focus on mammalian thioredoxin reductases – important selenoproteins with versatile functions publication-title: BBA-Gen. Subj. contributor: fullname: Arner – volume: 151 start-page: 658 year: 2008 end-page: 661 ident: bib0140 article-title: Identification of metabolites during biodegradation of pendimethalin in bioslurry reactor publication-title: J. Hazard. Mater. contributor: fullname: Sarma – volume: 64 start-page: 459 year: 1990 end-page: 465 ident: bib0190 article-title: 1, 2-Dichloropropane (Dcp) toxicity is correlated with dcp-induced glutathione (Gsh) depletion and is modulated by factors affecting intracellular gsh publication-title: Arch. Toxicol. contributor: fullname: Bellomo – volume: 76 start-page: 2940 year: 2010 end-page: 2945 ident: bib0080 article-title: Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated nocardioides sp strain SG-4G and its potential for use in enzymatic bioremediation publication-title: Appl. Environ. Microbiol. contributor: fullname: Oakeshott – volume: 72 start-page: 248 year: 1976 end-page: 254 ident: bib0130 article-title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding publication-title: Anal. Biochem. contributor: fullname: Bradford – volume: 4 start-page: 406 year: 1987 end-page: 425 ident: bib0125 article-title: The neighbor-Joining method – a new method for reconstructing phylogenetic trees publication-title: Mol. Biol. Evol. contributor: fullname: Nei – volume: 36 start-page: 37 year: 1998 end-page: 45 ident: bib0060 article-title: Developmental toxicity of carbendazim: comparison of no-observed-adverse-effect level and benchmark dose approach publication-title: Food Chem. Toxicol. contributor: fullname: Zapponi – volume: 25 start-page: 4876 year: 1997 end-page: 4882 ident: bib0120 article-title: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools publication-title: Nucleic Acids Res. contributor: fullname: Higgins – volume: 34 start-page: 73 year: 2002 end-page: 80 ident: bib0040 article-title: Stage-specific effects of the fungicide carbendazim on Sertoli cell microtubules in rat testis publication-title: Tissue Cell contributor: fullname: Hess – volume: 24 start-page: 371 year: 2007 end-page: 380 ident: bib0050 article-title: Modulation of antioxidant defense system by the environmental fungicide carbendazim in Leydig cells of rats publication-title: Reprod. Toxicol. contributor: fullname: Aruldhas – volume: 9 start-page: 18 year: 1995 end-page: 22 ident: bib0020 article-title: Degradation of organophosphorus and carbamate pesticides in soils – hplc determination publication-title: Biomed. Chromatogr. contributor: fullname: Mathur – volume: 434 start-page: 99 year: 2016 end-page: 106 ident: bib0200 article-title: Site-directed mutagenesis of bacterial cellulose synthase highlights sulfur-arene interaction as key to catalysis publication-title: Carbohydr. Res. contributor: fullname: Imai – volume: 8 year: 2013 ident: bib0085 article-title: Isolation and characterization of carbendazim-degrading rhodococcus erythropolis djl-11 publication-title: PLoS One contributor: fullname: Yang – volume: 39 start-page: 197 year: 2009 end-page: 204 ident: bib0185 article-title: 2-ABT-S-oxide detoxification by glutathione S-transferases A1-1, M1-1 and P1-1: implications for toxicity associated with zileuton publication-title: Xenobiotica; the Fate of Foreign Compounds in Biological Systems contributor: fullname: Macdonald – volume: 54 start-page: 122 year: 2015 end-page: 131 ident: bib0065 article-title: Parametric study on degradation of fungicide carbendazim in dilute aqueous solutions using nano TiO2 publication-title: Desalin Water Treat contributor: fullname: Wanchoo – volume: 21 start-page: 939 year: 2010 end-page: 946 ident: bib0090 article-title: Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim publication-title: Biodegradation contributor: fullname: Yu – volume: 321 start-page: 43 year: 1994 end-page: 56 ident: bib0030 article-title: Evaluation of carbendazim for gene mutations in the Salmonella/Ames plate-incorporation assay: the role of aminophenazine impurities publication-title: Mutat. Res. contributor: fullname: Reynolds – volume: 36 start-page: 4061 year: 1997 end-page: 4066 ident: bib0180 article-title: The CXXC motif: a rheostat in the active site publication-title: Biochemistry contributor: fullname: Raines – volume: 43 start-page: 125 year: 2015 end-page: 127 ident: bib0070 article-title: Research progress of carbendazim in China publication-title: J. Anhui Agri. contributor: fullname: Sun – volume: 53 start-page: 72 year: 2006 end-page: 76 ident: bib0100 article-title: Isolation and characterization of a carbendazim-degrading Rhodococcus sp djl-6 publication-title: Curr. Microbiol. contributor: fullname: Li – volume: 39 start-page: 400 year: 1991 end-page: 403 ident: bib0015 article-title: Residues of benomyl (determined as carbendazim) and captan in postharvest-treated pears in cold-storage publication-title: J. Agr. Food Chem. contributor: fullname: Deinzer – volume: 394 start-page: 644 year: 2009 end-page: 652 ident: bib0145 article-title: Genome sequences of escherichia coli B strains REL606 and BL21(DE3) publication-title: J. Mol. Biol. contributor: fullname: Kim – volume: 26 start-page: 228 year: 2008 end-page: 235 ident: bib0175 article-title: Functional diversity of cysteine residues in proteins and unique features of catalytic redox-active cysteines in thiol oxidoreductases publication-title: Mol. Cells contributor: fullname: Gladyshev – volume: 208 start-page: 84 year: 2016 ident: 10.1016/j.jhazmat.2017.02.007_bib0160 article-title: Role of cysteine residues in the redox-regulated oligomerization and nucleotide binding to EhRabX3 publication-title: Mol. Biochem. Parasitol. doi: 10.1016/j.molbiopara.2016.06.009 contributor: fullname: Chandra – volume: 434 start-page: 99 year: 2016 ident: 10.1016/j.jhazmat.2017.02.007_bib0200 article-title: Site-directed mutagenesis of bacterial cellulose synthase highlights sulfur-arene interaction as key to catalysis publication-title: Carbohydr. Res. doi: 10.1016/j.carres.2016.08.009 contributor: fullname: Sun – volume: 54 start-page: 122 year: 2015 ident: 10.1016/j.jhazmat.2017.02.007_bib0065 article-title: Parametric study on degradation of fungicide carbendazim in dilute aqueous solutions using nano TiO2 publication-title: Desalin Water Treat doi: 10.1080/19443994.2013.879081 contributor: fullname: Kaur – volume: 26 start-page: 228 year: 2008 ident: 10.1016/j.jhazmat.2017.02.007_bib0175 article-title: Functional diversity of cysteine residues in proteins and unique features of catalytic redox-active cysteines in thiol oxidoreductases publication-title: Mol. Cells doi: 10.1016/S1016-8478(23)13989-6 contributor: fullname: Fomenko – volume: 53 start-page: 72 year: 2006 ident: 10.1016/j.jhazmat.2017.02.007_bib0100 article-title: Isolation and characterization of a carbendazim-degrading Rhodococcus sp djl-6 publication-title: Curr. Microbiol. doi: 10.1007/s00284-005-0474-3 contributor: fullname: Xu – volume: 1 start-page: 68 year: 2003 ident: 10.1016/j.jhazmat.2017.02.007_bib0005 article-title: Degradation of carbendazim by UV/H2O2 investigated by kinetic modelling publication-title: Environ. Chem. Lett. doi: 10.1007/s10311-002-0010-7 contributor: fullname: Mazellier – year: 1994 ident: 10.1016/j.jhazmat.2017.02.007_bib0105 contributor: fullname: Holt – volume: 7 year: 2016 ident: 10.1016/j.jhazmat.2017.02.007_bib0165 article-title: Redox-active sensing by bacterial DksA transcription factors is determined by cysteine and zinc content publication-title: Mbio doi: 10.1128/mBio.02161-15 contributor: fullname: Crawford – volume: 81 start-page: 639 year: 2010 ident: 10.1016/j.jhazmat.2017.02.007_bib0095 article-title: Biodegradation of carbendazim by a novel actinobacterium Rhodococcus jialingiae djl-6-2 publication-title: Chemosphere doi: 10.1016/j.chemosphere.2010.08.040 contributor: fullname: Wang – volume: 36 start-page: 37 year: 1998 ident: 10.1016/j.jhazmat.2017.02.007_bib0060 article-title: Developmental toxicity of carbendazim: comparison of no-observed-adverse-effect level and benchmark dose approach publication-title: Food Chem. Toxicol. doi: 10.1016/S0278-6915(97)00116-6 contributor: fullname: Mantovani – volume: 64 start-page: 459 year: 1990 ident: 10.1016/j.jhazmat.2017.02.007_bib0190 article-title: 1, 2-Dichloropropane (Dcp) toxicity is correlated with dcp-induced glutathione (Gsh) depletion and is modulated by factors affecting intracellular gsh publication-title: Arch. Toxicol. doi: 10.1007/BF01977627 contributor: fullname: Imberti – volume: 9 start-page: 18 year: 1995 ident: 10.1016/j.jhazmat.2017.02.007_bib0020 article-title: Degradation of organophosphorus and carbamate pesticides in soils – hplc determination publication-title: Biomed. Chromatogr. doi: 10.1002/bmc.1130090104 contributor: fullname: Thapar – volume: 4 start-page: 406 year: 1987 ident: 10.1016/j.jhazmat.2017.02.007_bib0125 article-title: The neighbor-Joining method – a new method for reconstructing phylogenetic trees publication-title: Mol. Biol. Evol. contributor: fullname: Saitou – volume: 8 year: 2013 ident: 10.1016/j.jhazmat.2017.02.007_bib0085 article-title: Isolation and characterization of carbendazim-degrading rhodococcus erythropolis djl-11 publication-title: PLoS One contributor: fullname: Zhang – volume: 321 start-page: 43 year: 1994 ident: 10.1016/j.jhazmat.2017.02.007_bib0030 article-title: Evaluation of carbendazim for gene mutations in the Salmonella/Ames plate-incorporation assay: the role of aminophenazine impurities publication-title: Mutat. Res. doi: 10.1016/0165-1218(94)90119-8 contributor: fullname: Sarrif – volume: 27 start-page: 287 year: 2009 ident: 10.1016/j.jhazmat.2017.02.007_bib0045 article-title: Involvement of Sertoli cells in spermatogenic failure induced by carbendazim publication-title: Environ. Toxicol. Pharm. doi: 10.1016/j.etap.2008.11.006 contributor: fullname: Yu – volume: 186 start-page: 8599 year: 2014 ident: 10.1016/j.jhazmat.2017.02.007_bib0075 article-title: Kinetics of degradation of carbendazim by B-subtilis strains: possibility of in situ detoxification publication-title: Environ. Monit. Assess. doi: 10.1007/s10661-014-4027-8 contributor: fullname: Salunkhe – volume: 38 start-page: 155 year: 2016 ident: 10.1016/j.jhazmat.2017.02.007_bib0150 article-title: Critical reflections on synthetic gene design for recombinant protein expression publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2016.07.004 contributor: fullname: Parret – volume: 62 start-page: 716 year: 2012 ident: 10.1016/j.jhazmat.2017.02.007_bib0110 article-title: Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species publication-title: Int. J. Syst. Evolut. Microbiol. doi: 10.1099/ijs.0.038075-0 contributor: fullname: Kim – volume: 1790 start-page: 495 year: 2009 ident: 10.1016/j.jhazmat.2017.02.007_bib0195 article-title: Focus on mammalian thioredoxin reductases – important selenoproteins with versatile functions publication-title: BBA-Gen. Subj. doi: 10.1016/j.bbagen.2009.01.014 contributor: fullname: Arner – volume: 30 start-page: 2725 year: 2013 ident: 10.1016/j.jhazmat.2017.02.007_bib0115 article-title: MEGA6: molecular evolutionary genetics analysis version 6.0 publication-title: Mol. Biol. Evolut. doi: 10.1093/molbev/mst197 contributor: fullname: Tamura – volume: 404 start-page: 902 year: 2010 ident: 10.1016/j.jhazmat.2017.02.007_bib0170 article-title: Cysteine function governs its conservation and degeneration and restricts its utilization on protein surfaces publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2010.09.027 contributor: fullname: Marino – volume: 72 start-page: 248 year: 1976 ident: 10.1016/j.jhazmat.2017.02.007_bib0130 article-title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding publication-title: Anal. Biochem. doi: 10.1016/0003-2697(76)90527-3 contributor: fullname: Bradford – volume: 39 start-page: 400 year: 1991 ident: 10.1016/j.jhazmat.2017.02.007_bib0015 article-title: Residues of benomyl (determined as carbendazim) and captan in postharvest-treated pears in cold-storage publication-title: J. Agr. Food Chem. doi: 10.1021/jf00002a035 contributor: fullname: Kiigemagi – volume: 24 start-page: 371 year: 2007 ident: 10.1016/j.jhazmat.2017.02.007_bib0050 article-title: Modulation of antioxidant defense system by the environmental fungicide carbendazim in Leydig cells of rats publication-title: Reprod. Toxicol. doi: 10.1016/j.reprotox.2007.03.010 contributor: fullname: Rajeswary – volume: 43 start-page: 125 year: 2015 ident: 10.1016/j.jhazmat.2017.02.007_bib0070 article-title: Research progress of carbendazim in China publication-title: J. Anhui Agri. contributor: fullname: Wei – volume: 17 start-page: 687 year: 2003 ident: 10.1016/j.jhazmat.2017.02.007_bib0035 article-title: Down regulation of humoral immunity in chickens due to carbendazim publication-title: Toxicol. in Vitro doi: 10.1016/S0887-2333(03)00103-6 contributor: fullname: Singhal – volume: 151 start-page: 658 year: 2008 ident: 10.1016/j.jhazmat.2017.02.007_bib0140 article-title: Identification of metabolites during biodegradation of pendimethalin in bioslurry reactor publication-title: J. Hazard. Mater. doi: 10.1016/j.jhazmat.2007.06.039 contributor: fullname: Ramakrishna – volume: 39 start-page: 197 year: 2009 ident: 10.1016/j.jhazmat.2017.02.007_bib0185 article-title: 2-ABT-S-oxide detoxification by glutathione S-transferases A1-1, M1-1 and P1-1: implications for toxicity associated with zileuton publication-title: Xenobiotica; the Fate of Foreign Compounds in Biological Systems doi: 10.1080/00498250802651976 contributor: fullname: Joshi – volume: 36 start-page: 4061 year: 1997 ident: 10.1016/j.jhazmat.2017.02.007_bib0180 article-title: The CXXC motif: a rheostat in the active site publication-title: Biochemistry doi: 10.1021/bi9628580 contributor: fullname: Chivers – volume: 32 start-page: 291 year: 1975 ident: 10.1016/j.jhazmat.2017.02.007_bib0135 article-title: Effects of selected sulfhydryl inhibitors on nonhistone chromosomal proteins of HeLa cells publication-title: Oncology doi: 10.1159/000225079 contributor: fullname: Knock – volume: 25 start-page: 4876 year: 1997 ident: 10.1016/j.jhazmat.2017.02.007_bib0120 article-title: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools publication-title: Nucleic Acids Res. doi: 10.1093/nar/25.24.4876 contributor: fullname: Thompson – volume: 34 start-page: 73 year: 2002 ident: 10.1016/j.jhazmat.2017.02.007_bib0040 article-title: Stage-specific effects of the fungicide carbendazim on Sertoli cell microtubules in rat testis publication-title: Tissue Cell doi: 10.1016/S0040-8166(02)00006-X contributor: fullname: Nakai – volume: 76 start-page: 2940 year: 2010 ident: 10.1016/j.jhazmat.2017.02.007_bib0080 article-title: Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated nocardioides sp strain SG-4G and its potential for use in enzymatic bioremediation publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.02990-09 contributor: fullname: Pandey – volume: 50 start-page: 649 year: 2003 ident: 10.1016/j.jhazmat.2017.02.007_bib0010 article-title: Photochemical behaviour of carbendazim in aqueous solution publication-title: Chemosphere doi: 10.1016/S0045-6535(02)00620-3 contributor: fullname: Boudina – volume: 394 start-page: 644 year: 2009 ident: 10.1016/j.jhazmat.2017.02.007_bib0145 article-title: Genome sequences of escherichia coli B strains REL606 and BL21(DE3) publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2009.09.052 contributor: fullname: Jeong – volume: 172 start-page: 84 year: 2009 ident: 10.1016/j.jhazmat.2017.02.007_bib0055 article-title: Effect of carbendazim and pencycuron on soil bacterial community publication-title: J. Hazard. Mater. doi: 10.1016/j.jhazmat.2009.06.142 contributor: fullname: Wang – volume: 8 start-page: 283 year: 2013 ident: 10.1016/j.jhazmat.2017.02.007_bib0155 article-title: Diverse functional roles of reactive cysteines publication-title: ACS Chem. Biol. doi: 10.1021/cb3005269 contributor: fullname: Pace – volume: 48 start-page: 251 year: 2000 ident: 10.1016/j.jhazmat.2017.02.007_bib0025 article-title: Impact of the fungicide carbendazim in freshwater microcosms. II. Zooplankton, primary producers and final conclusions publication-title: Aquat. Toxicol. doi: 10.1016/S0166-445X(99)00037-5 contributor: fullname: Van den Brink – volume: 21 start-page: 939 year: 2010 ident: 10.1016/j.jhazmat.2017.02.007_bib0090 article-title: Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim publication-title: Biodegradation doi: 10.1007/s10532-010-9353-0 contributor: fullname: Fang |
| SSID | ssj0001754 |
| Score | 2.4375756 |
| Snippet | •Strain SD-4 was the first MBC-degrading strain isolated from the genus of Mycobacterium.•The codon of mheI was optimized to realize its soluble expression in... A novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC) degrading strain SD-4 was isolated and identified preliminarily as Mycobacterium sp.... |
| SourceID | proquest crossref pubmed elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 55 |
| SubjectTerms | Benzimidazoles - metabolism Carbamates - metabolism Carbendazim Escherichia coli Expression Genes, Bacterial Hydrolases - chemistry Hydrolases - genetics Hydrolases - metabolism Key amino acid sites mheI Mycobacterium - enzymology Mycobacterium - genetics Mycobacterium - isolation & purification Mycobacterium sp. SD-4 |
| Title | Identification of the key amino acid sites of the carbendazim hydrolase (MheI) from a novel carbendazim-degrading strain Mycobacterium sp. SD-4 |
| URI | https://dx.doi.org/10.1016/j.jhazmat.2017.02.007 https://www.ncbi.nlm.nih.gov/pubmed/28242529 https://search.proquest.com/docview/1872870764 |
| Volume | 331 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB51txc4ICiv5VEZiQMcshsn9jo5VoVqC2wvLVJvlp_arNhktdkFtQf-An-ZcR6lHCokjklsxfLY833jeRjgrU24p5rbyHi0VVnGXaQtzyOqUBd6NXWZDx7d-dl09pV9uuSXe3Dc58KEsMpO97c6vdHW3ZtJN5uTdVFMzoNTD-GW0VByHYniAPYRjhgbwv7R6efZ2Y1CRoRsq0gFJwB2-JPIM1mOlwt1jdwwBHmJtnqnuAui7qKgDRSdPIQHHYckR-0wH8GeKw_g_q3Kggcw-KJ-PIZfbRqu787lSOUJ8j2C-5aoVVFWRJnCkuA_rvtvRm20QzP9uliRxZXdoOVbO_JuvnCn70nIRSGKlNV39-12y8iGkhMBBUnd3DlB5lcGFUVTCHq3IvV6TM4_ROwJXJx8vDieRd0NDJFBu3IbiSw2WcK0sdzqlNtUxynjmfMJUwJln6TGosXGzdTmNHfYVof8Q4U2txA5TZ_CsKxK9xyISVNcFDH1yiFD01QLm9uY-0QhxePUjmDcz7lct3U2ZB-AtpSdkGQQkowTiUIaQdZLRv61YCRiwb-6vuklKXEzBQ-JKl21qyXNRHD8iikbwbNWxDejQdsU9VuSv_j_H7-Ee-GpiTTjr2C43ezca-Q0W30Ig_FPetit3N80gPZE |
| link.rule.ids | 315,786,790,4521,24144,27955,27956,45618,45712 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LbxMxEB616QE4ICiv8DQSBzhsst5dx7vHqlAlNMmlQerN8lPZiOxG2QTU_gn-MuN9lHKokLiubdny2DPft_MwwAcTMUcVM4F2yFWTlNlAGZYFVKIudHJkU-c9urP5aPwt-XrJLg_gtMuF8WGVre5vdHqtrdsvw3Y3h5s8H154px6a24T6kusIFA_hKGGcRj04Opmcj-c3ChktZFNFyjsBcMCfRJ7harBaymvEhj7IizfVO_ldJuouCFqborNH8LDFkOSkWeZjOLDFMTy4VVnwGA6n8ucT-NWk4br2vxwpHUG8R_DeErnOi5JInRvi_cdV16blVlmk6df5miyvzBaZb2XJx9nSTj4Rn4tCJCnKH_b77Z6B8SUnvBUkVf3mBJldaVQUdSHo_ZpUmwG5-BwkT2Fx9mVxOg7aFxgCjbxyF_A01GmUKG2YUTEzsQrjhKXWRYnkKPso1gYZG9Mjk9HMYl_l8w8lcm7OMxo_g15RFvYFEB3HeChC6qRFhKao4iYzIXORRIjHqOnDoNtzsWnqbIguAG0lWiEJLyQRRgKF1Ie0k4z468AItAX_Gvq-k6TAy-Q9JLKw5b4SNOXe8ctHSR-eNyK-WQ1yU9RvUfby_yd-B_fGi9lUTCfz81dw37fUUWfsNfR22719g_hmp9625_c3HkH4NA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Identification+of+the+key+amino+acid+sites+of+the+carbendazim+hydrolase+%28MheI%29+from+a+novel+carbendazim-degrading+strain+Mycobacterium+sp.+SD-4&rft.jtitle=Journal+of+hazardous+materials&rft.au=Zhang%2C+Yingkun&rft.au=Wang%2C+Hui&rft.au=Wang%2C+Xiang&rft.au=Hu%2C+Bo&rft.date=2017-06-05&rft.eissn=1873-3336&rft.volume=331&rft.spage=55&rft_id=info:doi/10.1016%2Fj.jhazmat.2017.02.007&rft_id=info%3Apmid%2F28242529&rft.externalDocID=28242529 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0304-3894&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0304-3894&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0304-3894&client=summon |