B1 and TRPV-1 receptor genes and their relationship to hyperalgesia following spinal cord injury
Laboratory investigation of pain behavior following spinal cord injury. To explore changes in the spinal cord expression of nociceptive genes following spinal cord injury (SCI) as they relate to the manifestation of pain behavior in rats. Neuropathic pain following SCI is common, disabling, and larg...
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Published in | Spine (Philadelphia, Pa. 1976) Vol. 31; no. 24; p. 2778 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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United States
15.11.2006
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Abstract | Laboratory investigation of pain behavior following spinal cord injury.
To explore changes in the spinal cord expression of nociceptive genes following spinal cord injury (SCI) as they relate to the manifestation of pain behavior in rats.
Neuropathic pain following SCI is common, disabling, and largely untreatable. In peripheral nerve injury models, bradykinin B1 and vanilloid 1 (TRPV-1) receptor activity is associated with neuropathic pain behavior. We sought to examine the role of these gene products in SCI-mediated pain.
Rats were subjected to SCI using the MASCIS impactor. Animals were tested preinjury and at regular intervals postinjury for the appearance of thermal hyperalgesia using a hind limb withdrawal latency test. The expression of B1 and TRPV-1 genes was assessed using real-time polymerase chain reaction. Immunohistochemistry was used to localize the B1 and TRPV-1 receptors within the spinal cord.
Greater than twofold increases in the expression of the B1 and TRPV-1 genes were detected in the injured region of the spinal cord in animals exhibiting hyperalgesia compared with animals with SCI that did not display hyperalgesia. Immunohistochemical staining revealed that both receptor types were largely localized to the dorsal horn. Staining for TRPV-1 receptors decreased while that for B1 receptors increased in all of the injured animals when compared with sham-operated controls.
B1 and TRPV-1 receptor genes are overexpressed in the injured spinal cord of animals manifesting thermal hyperalgesia following SCI compared with similarly injured animals without hyperalgesia. This finding is consistent with past work regarding the role of these receptors in nociception and indicates that ongoing modifiable processes are occurring in the spinal cord that lead to clinical pain syndromes. |
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AbstractList | Laboratory investigation of pain behavior following spinal cord injury.
To explore changes in the spinal cord expression of nociceptive genes following spinal cord injury (SCI) as they relate to the manifestation of pain behavior in rats.
Neuropathic pain following SCI is common, disabling, and largely untreatable. In peripheral nerve injury models, bradykinin B1 and vanilloid 1 (TRPV-1) receptor activity is associated with neuropathic pain behavior. We sought to examine the role of these gene products in SCI-mediated pain.
Rats were subjected to SCI using the MASCIS impactor. Animals were tested preinjury and at regular intervals postinjury for the appearance of thermal hyperalgesia using a hind limb withdrawal latency test. The expression of B1 and TRPV-1 genes was assessed using real-time polymerase chain reaction. Immunohistochemistry was used to localize the B1 and TRPV-1 receptors within the spinal cord.
Greater than twofold increases in the expression of the B1 and TRPV-1 genes were detected in the injured region of the spinal cord in animals exhibiting hyperalgesia compared with animals with SCI that did not display hyperalgesia. Immunohistochemical staining revealed that both receptor types were largely localized to the dorsal horn. Staining for TRPV-1 receptors decreased while that for B1 receptors increased in all of the injured animals when compared with sham-operated controls.
B1 and TRPV-1 receptor genes are overexpressed in the injured spinal cord of animals manifesting thermal hyperalgesia following SCI compared with similarly injured animals without hyperalgesia. This finding is consistent with past work regarding the role of these receptors in nociception and indicates that ongoing modifiable processes are occurring in the spinal cord that lead to clinical pain syndromes. |
Author | Gerovac, Tiffany A Vemuganti, Raghu Miranpuri, Gurwattan S Resnick, Daniel K Türeyen, Kudret Turner, Nicholas A Satriotomo, Irawan DomBourian, Melkon G Miletic, Vjekoslav |
Author_xml | – sequence: 1 givenname: Melkon G surname: DomBourian fullname: DomBourian, Melkon G organization: Department of Neurological Surgery, University of Wisconsin Medical School, Madison, WI 53792, USA – sequence: 2 givenname: Nicholas A surname: Turner fullname: Turner, Nicholas A – sequence: 3 givenname: Tiffany A surname: Gerovac fullname: Gerovac, Tiffany A – sequence: 4 givenname: Raghu surname: Vemuganti fullname: Vemuganti, Raghu – sequence: 5 givenname: Gurwattan S surname: Miranpuri fullname: Miranpuri, Gurwattan S – sequence: 6 givenname: Kudret surname: Türeyen fullname: Türeyen, Kudret – sequence: 7 givenname: Irawan surname: Satriotomo fullname: Satriotomo, Irawan – sequence: 8 givenname: Vjekoslav surname: Miletic fullname: Miletic, Vjekoslav – sequence: 9 givenname: Daniel K surname: Resnick fullname: Resnick, Daniel K |
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Snippet | Laboratory investigation of pain behavior following spinal cord injury.
To explore changes in the spinal cord expression of nociceptive genes following spinal... |
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SubjectTerms | Animals Contusions - complications Contusions - physiopathology Gene Expression Regulation Hindlimb - innervation Hot Temperature - adverse effects Hyperalgesia - etiology Hyperalgesia - genetics Hyperalgesia - physiopathology Inflammation Laminectomy Male Models, Animal Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - physiology Nociceptors - physiology Pain Measurement Rats Rats, Sprague-Dawley Reaction Time Receptor, Bradykinin B1 - biosynthesis Receptor, Bradykinin B1 - genetics Receptor, Bradykinin B1 - physiology Reverse Transcriptase Polymerase Chain Reaction Spinal Cord Injuries - complications Spinal Cord Injuries - physiopathology TRPV Cation Channels - biosynthesis TRPV Cation Channels - genetics TRPV Cation Channels - physiology |
Title | B1 and TRPV-1 receptor genes and their relationship to hyperalgesia following spinal cord injury |
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