Enantioselective Esterification of Ibuprofen by a Novel Thermophilic Biocatalyst: APE1547

• Published in: Biotechnology and bioprocess engineering Vol. 16; no. 4; pp. 638 - 644
• Main Authors: Zhao, Dan-tong, Shenyang University, Shenyang, China, Xun, Er-na, Jilin University, Changchun, China, Wang, Jia-xin, Jilin University, Changchun, China, Wang, Ren, Jilin University, Changchun, China, Wei, Xiao-fei, Jilin University, Changchun, China, Wang, Lei, Jilin University, Changchun, China, Wang, Zhi, Jilin University, Changchun, China
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.08.2011; Springer Nature B.V; 한국생물공학회
• Subjects: Aeropyrum pernix; Alcohol; Analysis; Biocatalysts; Biotechnology; Catalysts; Celite; Chemistry; Chemistry and Materials Science; E coli; Enantiomers; enantioselectivity; Energy consumption; Engineering; Enzymatic activity; enzyme activity; Enzymes; esterase; ESTERIFICACION; ESTERIFICATION; High temperature; Ibuprofen; Industrial and Production Engineering; Laboratories; Nonsteroidal anti-inflammatory drugs; Organic solvents; Pharmaceuticals; Reagents; Research Paper; Solvents; Studies; Water activity; Water temperature; 생물공학; enantioselectivity; ibuprofen; esterification; enzyme activity
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-011-0007-9

The enantioselective esterification of ibuprofen catalyzed by a novel thermophilic esterase (APE1547) from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents. The effects of acyl acceptor, substrate ratio, organic solvent, temperature, and water activity were investigated. Under optimum conditions, the highest enantioselectivity (E = 38.1) was obtained with a higher enzyme activity (216.5 μmol/g/h). Celites were added into the reaction mixture to remove the water produced in the esterification. The reaction achieved its equilibrium in approximately 96 h with a conversion of 57 and 99% (ee) of the un-reacted (S)-ibuprofen obtained.