Best practices in cell culture: an overview

• Published in: In vitro cellular & developmental biology. Animal Vol. 53; no. 8; pp. 669 - 672
• Main Authors: Baust, John M., Buehring, Gertrude Case, Campbell, Lia, Elmore, Eugene, Harbell, John W., Nims, Raymond W., Price, Paul, Reid, Yvonne A., Simione, Frank
• Format: Journal Article
• Language: English
• Published: New York Springer Science & Business Media LLC 01.09.2017; Springer US; Society for In Vitro Biology
• Subjects: Animal Genetics and Genomics; Animals; BEST PRACTICES IN CELL CULTURE; Biomedical and Life Sciences; Biotechnology; Cancer; Cell Biology; Cell Culture; Cell Culture Techniques - methods; Cell Culture Techniques - trends; Cell Line; Cell lines; Contamination; Cryopreservation; Cryopreservation - methods; Culture Media; Developmental Biology; Gene expression; Laboratories; Life Sciences; Names; Scientists; Shipping; Stem Cells; Thawing; Overview; Best practices in cell culture
• ISSN: 1071-2690; 1543-706X
• DOI: 10.1007/s11626-017-0177-7

This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells.