Blue native PAGE as a useful method for the analysis of the assembly of distinct combinations of nicotinic acetylcholine receptor subunits

• Published in: Journal of receptors and signal transduction Vol. 19; no. 1-4; p. 493
• Main Authors: Nicke, A, Rettinger, J, Mutschler, E, Schmalzing, G
• Format: Journal Article
• Language: English
• Published: England 01.01.1999
• Subjects: Animals; Electrophoresis, Polyacrylamide Gel - methods; Female; In Vitro Techniques; Muscles - metabolism; Oocytes - metabolism; Protein Conformation; Rats; Receptors, Nicotinic - chemistry; Receptors, Nicotinic - genetics; Receptors, Nicotinic - metabolism; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Xenopus laevis

Oligomerization of complete and incomplete combinations of rat muscle-type nicotinic acetylcholine receptor (nAChR) subunits in Xenopus oocytes was studied by blue native PAGE and compared with acetylcholine-activated current in these cells. The rank order of expression level judged by current was a...