Use of mutants to indicate factors prerequisite for penetration of Colletotrichum lagenarium by appressoria

• Published in: Physiological and molecular plant pathology Vol. 32; no. 1; pp. 177 - 184
• Main Authors: Katoh, Masayasu, Hirose, Izumi, Kubo, Yasuyuki, Hikichi, Yasufumi, Kunoh, Hitoshi, Furusawa, Iwao, Shishiyama, Jiko
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 1988; Elsevier
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; Host agent relation; Phytopathogen; Adhesion; Fungi; Morphogenesis; Colletotrichum lagenarium; Transmission electron microscopy; Penetration; Ultrastructure; Fungi Imperfecti; Mutation; Appressorium; Thallophyta; Non existence
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1016/S0885-5765(88)80014-6

Three mutants of Colletotrichum lagenarium, with appressoria that could not penetrate nitrocellulose membranes or cell walls of the host plant, were obtained by mutagenic treatment. Appressoria of these mutants were identical in shape and colour to those of the parent strain. Scanning electron microscopy revealed that one mutant (83182) formed a penetration peg at the basal part of the appressoria and that the other mutants (82335 and 83348) did not form a penetration peg. The latter mutants are considered to have a defect in morphogenesis of the penetration peg. Application of Czapek liquid medium to mutant 83182 after appressorium formation partially restored appressorial penetration without halo formation; this mutant synthesized a protein, P-95, which was closely related to cellulose degrading enzymes. These results suggest that mutant 83182 is deficient in a secretion or translocation system for these enzymes.