Changes in lipolysis and hormone-sensitive lipase expression caused by procyanidins in 3T3-L1 adipocytes

• Published in: International Journal of Obesity Vol. 24; no. 3; pp. 319 - 324
• Main Authors: Ardevol, A, Blade, C, Salvado, M.J, Arola, L
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 01.03.2000; Nature Publishing Group
• Subjects: 3T3 Cells; Adipocytes; Adipocytes - drug effects; Adipocytes - enzymology; analysis; Animals; Biflavonoids; Biological and medical sciences; catechin; Catechin - pharmacology; Cell Survival; Cocoa; Dehydrogenase; Dehydrogenases; drug effects; Enzymes; enzymology; epicatechin; Gene Expression; Gene Expression - drug effects; genetics; Glycerol; Glycerol - metabolism; glycerol-3-phosphate dehydrogenase; Glycerolphosphate Dehydrogenase; Glycerolphosphate Dehydrogenase - metabolism; hydrolysis; Kinases; Kinetics; Lipids; Lipolysis; Medical sciences; messenger RNA; Metabolic diseases; Metabolism; Mice; Molecular weight; Obesity; Penicillin; pharmacology; Phenols; Phenols - pharmacology; Phosphorylation; Polymerization; Proanthocyanidins; procyanidins; Proteins; RNA, Messenger; RNA, Messenger - analysis; Sterol Esterase; Sterol Esterase - genetics; Sterol Esterase - metabolism; toxicity; triacylglycerols; Triglycerides; Triglycerides - analysis; viability; Wine; Wine - analysis; Wines; Human; Adipocyte; Cell culture; Obesity; Catechin; Glycerol-3-phosphate dehydrogenase; Enzyme; Nutrition disorder; Alcohol; In vitro; Biological activity; Lipolysis; Enzymatic activity; Oxidoreductases; Nutritional status; Comparative study
• DOI: 10.1038/sj.ijo.0801130
• ISSN: 0307-0565

OBJECTIVE: To find out whether lipid stores are influenced by phenolic compounds in wine. DESIGN: Differentiated 3T3-L1 cells were treated with catechin, epicatechin or procyanidin extracts with different degrees of polymerization at 150 micromolar for different periods of time (0.5-24 h). SUBJECTS: Cell line 3T3-L1. MEASUREMENTS: Cellular viability, glycerol-3-phosphate dehydrogenase activity, glycerol release in the medium, HSL mRNA levels, triacylglycerols and protein. RESULTS: Catechin, epicatechin and procyanidin extracts were not toxic for the 3T3-L1 cells in the conditions assayed. Glycerol-3-phosphate dehydrogenase activity was markedly decreased by 150 micromolar procyanidin extracts. The release of glycerol into the medium was increased in 150 micromolar procyanidin extract-treated cells and reached a plateau after 15 h exposure. Procyanidins caused a time-dependent reduction in the HSL mRNA levels. CONCLUSIONS: These results suggest that procyanidins from grape and wine affect lipid metabolism whilst their monomers (catechin and epicatechin) do not. This effect is more pronounced when the degree of polymerization is higher. Procyanidin extracts cause a time-dependent reduction in the HSL mRNA levels, inhibit triacylglycerol synthesis and also favour triacylglycerol hydrolysis until the HSL mRNA had reached very low levels.