Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems

ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to m...

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Published inBrazilian Archives of Biology and Technology Vol. 60
Main Authors Reali, Catiusca, Pilz Júnior, Harry Luiz, Meile, Jean Christophe, Galindo, Sabine Shorr, Fiuza, Lidia Mariana
Format Journal Article
LanguageEnglish
Published Instituto de Tecnologia do Paraná (Tecpar) 01.01.2017
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Abstract ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be obtained by cultural methods. The aim of this paper is to review the functional groups related to agroecosystems and to indicate the critical choice of DNA primers pairs and targeted DNA regions that may be used in PCR-based methods such as the DGGE technique in order to evaluate the microbial communities in a variety of environments.
AbstractList ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be obtained by cultural methods. The aim of this paper is to review the functional groups related to agroecosystems and to indicate the critical choice of DNA primers pairs and targeted DNA regions that may be used in PCR-based methods such as the DGGE technique in order to evaluate the microbial communities in a variety of environments.
Author Reali, Catiusca
Pilz Júnior, Harry Luiz
Meile, Jean Christophe
Fiuza, Lidia Mariana
Galindo, Sabine Shorr
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  surname: Reali
  fullname: Reali, Catiusca
  organization: Universidade do Vale do Rio dos Sinos, Brazil
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  givenname: Lidia Mariana
  surname: Fiuza
  fullname: Fiuza, Lidia Mariana
  organization: Instituto Rio Grandense do Arroz, Brasil
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Snippet ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies...
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SubjectTerms agroecosystems
bacteria
DGGE
functional genes
metagenomics
microbial ecology
Title Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems
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