A microfluidic cytometer for white blood cell analysis

Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype o...

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Published inCytometry. Part A Vol. 99; no. 11; pp. 1107 - 1113
Main Authors Peng, Tao, Su, Xinyue, Cheng, Xingzhi, Wei, Zewen, Su, Xuantao, Li, Qin
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.11.2021
Wiley Subscription Services, Inc
Subjects
3D hydrodynamic focusing
airborne microlens
Blood
Classification
Cytometry
Erythrocytes
Flow Cytometry
Hydrodynamics
Leukocytes
Microchannels
Microfluidic Analytical Techniques
microfluidic cytometer
Microfluidics
Microlenses
Optical fibers
Portable equipment
Sheaths
white blood cells classification
airborne microlens
3D hydrodynamic focusing
white blood cells classification
microfluidic cytometer
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Abstract Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype of microfluidic cytometer which integrates a three‐dimensional hydrodynamic focusing system and an on‐chip optical system to count and classify white blood cells. By adjusting the flow speed of sheath flow and sample flow, the blood cells can be horizontally and vertically focused in the center of microchannel. Optical fibers and on‐chip microlens are embedded for the excitation and detection of single‐cell. The microfluidic chip was validated by classifying white blood cells from clinical blood samples.
AbstractList Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype of microfluidic cytometer which integrates a three‐dimensional hydrodynamic focusing system and an on‐chip optical system to count and classify white blood cells. By adjusting the flow speed of sheath flow and sample flow, the blood cells can be horizontally and vertically focused in the center of microchannel. Optical fibers and on‐chip microlens are embedded for the excitation and detection of single‐cell. The microfluidic chip was validated by classifying white blood cells from clinical blood samples.
Abstract Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype of microfluidic cytometer which integrates a three‐dimensional hydrodynamic focusing system and an on‐chip optical system to count and classify white blood cells. By adjusting the flow speed of sheath flow and sample flow, the blood cells can be horizontally and vertically focused in the center of microchannel. Optical fibers and on‐chip microlens are embedded for the excitation and detection of single‐cell. The microfluidic chip was validated by classifying white blood cells from clinical blood samples.
Author Cheng, Xingzhi
Peng, Tao
Su, Xinyue
Wei, Zewen
Su, Xuantao
Li, Qin
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Issue 11
Keywords airborne microlens
3D hydrodynamic focusing
white blood cells classification
microfluidic cytometer
Language English
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Notes Funding information
Tao Peng and Xinyue Su contributed equally to this study.
Shandong Provincial Key Research and Development Program Major Scientific and Technological Innovation Project, Grant/Award Number: 2019JZZY011016; Beijing Institute of Technology Research Fund Program for Young Scholars, Grant/Award Number: 3160011181907; Biological & Medical Engineering Core Facilities, Beijing Institute of Technology; National Natural Science Foundation of China, Grant/Award Numbers: 61975017, 62074016
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Snippet Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to be...
Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point-of-care testing remains to be...
Abstract Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to...
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SubjectTerms 3D hydrodynamic focusing
airborne microlens
Blood
Classification
Cytometry
Erythrocytes
Flow Cytometry
Hydrodynamics
Leukocytes
Microchannels
Microfluidic Analytical Techniques
microfluidic cytometer
Microfluidics
Microlenses
Optical fibers
Portable equipment
Sheaths
white blood cells classification
Title A microfluidic cytometer for white blood cell analysis
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https://www.ncbi.nlm.nih.gov/pubmed/34369647
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