DNA pooling analysis of ADHD and genes regulating vesicle release of neurotransmitters

• Published in: American journal of medical genetics. Part B, Neuropsychiatric genetics Vol. 139B; no. 1; pp. 33 - 37
• Main Authors: Brookes, K.J., Knight, J., Xu, X., Asherson, P.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 05.11.2005
• Subjects: ADHD synaptic components; association; Attention Deficit Disorder with Hyperactivity - genetics; Attention Deficit Disorder with Hyperactivity - metabolism; Cytoplasmic Vesicles - secretion; DNA - metabolism; DNA pooling; Female; Gene Frequency; Gene Pool; Genetic Markers; Genetic Predisposition to Disease; Humans; Neurotransmitter Agents - metabolism; Polymorphism, Single Nucleotide; Synapses - metabolism
• ISSN: 1552-4841; 1552-485X
• DOI: 10.1002/ajmg.b.30216

ADHD is one of the most prevalent, and heritable behavioural disorders in childhood. Genetic associations have been reported with polymorphic variants within or near to dopamine pathway genes. Recently snap‐25 has also shown association with ADHD in several datasets. We therefore investigated other genes that produce proteins that interact with SNAP‐25 in the mechanism of vesicular release of neurotransmitters at the synapse. A total of 106 SNPs were screened for minor allele frequency greater than 5% and 61 SNPs selected for analysis in DNA pools made up from an ADHD clinical sample of DSM‐IV combined type probands (n = 180) and a control sample of 90 males and 90 females. Initial screening identified several SNPs that showed allele frequency differences of 5% or more. One SNP in the synaptophysin gene showed suggestive evidence of association following case‐control and TDT analysis and warrants further investigation. © 2005 Wiley‐Liss, Inc.