Unbiased method for spectral analysis of cells with great diversity of autofluorescence spectra - Roet, Janna E. G.; Mikula, Aleksandra M.; Kok, Michael; Chadick, Cora H.; Garcia Vallejo, Juan J.; Roest, Henk P.; Laan, Luc J. W.; Winde, Charlotte M.; Mebius, Reina E. (Cytometry. Part A, 2024)
Keywords: autofluorescence; Biological properties; Biological samples; Biomarkers; Cell activation; Cell suspensions; Clustering; data analysis; dimensional reduction; Emission analysis; Emission spectra; Emissions; Excitation spectra; Flow cytometry; Flow Cytometry - methods; Fluorescence; Fluorescent Dyes - chemistry; Heterogeneity; Humans; In vitro methods and tests; Leukocytes (mononuclear); Light emission; Metabolic rate; Peripheral blood mononuclear cells; Phenotyping; Spectral analysis; spectral flow cytometry; spectral unmixing; Spectrometry, Fluorescence - methods; Spectrum analysis; unbiased clustering; Wavelengths; Workflow; data analysis; spectral unmixing; dimensional reduction; autofluorescence; unbiased clustering; spectral flow cytometry; heterogeneity