Urine RNA Processing in a Clinical Setting: Comparison of 3 Protocols

• Published in: Female pelvic medicine & reconstructive surgery Vol. 25; no. 3; p. 247
• Main Authors: Bradley, Megan S, Boudreau, Marie-Helene, Grenier, Carole, Huang, Zhiqing, Murphy, Susan K, Siddiqui, Nazema Y
• Format: Journal Article
• Language: English
• Published: United States 01.05.2019
• Subjects: Female; Genetic Techniques - instrumentation; Genetic Techniques - standards; Humans; Reagent Kits, Diagnostic - standards; Real-Time Polymerase Chain Reaction - instrumentation; Real-Time Polymerase Chain Reaction - standards; RNA - isolation & purification; RNA - urine

The objective of this study was to compare quantitative and qualitative RNA extraction results from clinical voided urine samples between 3 commercially available extraction protocols. For phase 1, fresh voided urine samples from 10 female subjects were collected and processed in clinic and transpor...