Innovative fluorescent probes for in vivo visualization of biomolecules in living Caenorhabditis elegans

Caenorhabditis elegans (C. elegans) as a well‐established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high‐reproductive rate, well‐defined anatomy, and conserved genome, has made C....

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Published inCytometry. Part A Vol. 99; no. 6; pp. 560 - 574
Main Authors Wang, Chunxia, Xia, Chujie, Zhu, Yi, Zhang, Huimin
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.06.2021
Wiley Subscription Services, Inc
Subjects
Aging
Biomolecules
Biophysics
Body size
Caenorhabditis elegans
Cell size
DNA probes
Fluorescent indicators
fluorescent probes
Genomes
Immunology
Labeling
Life cycles
Nematodes
Nervous system
Optical analysis
optical imaging
Organisms
Probes
Qualitative analysis
Quantitative analysis
Worms
Caenorhabditis elegans
biomolecules
optical imaging
fluorescent probes
Online AccessGet full text
ISSN1552-4922
1552-4930
1552-4930
DOI10.1002/cyto.a.24325

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Abstract Caenorhabditis elegans (C. elegans) as a well‐established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high‐reproductive rate, well‐defined anatomy, and conserved genome, has made C. elegans one of the most successful multicellular model organisms. Discoveries obtained from the C. elegans model have made great contributions to research fields such as development, aging, biophysics, immunology, and neuroscience. Because of its transparent body and giant cell size, C. elegans is also an ideal subject for high resolution and high‐throughput optical imaging and analysis. During the past decade, great advances have been made to develop biomolecule‐targeting techniques for noninvasive optical imaging. These novel technologies expanded the toolbox for qualitative and quantitative analysis of biomolecules in C. elegans. In this review, we summarize recently developed fluorescent probes or labeling techniques for visualizing biomolecules at the cellular, subcellular or molecular scale by using C. elegans as the major model organism or designed specifically for the applications in C. elegans. Combining the technological advantages of the C. elegans model with the novel fluorescent labeling techniques will provide new horizons for high‐efficiency quantitative optical analysis in live organisms.
AbstractList Caenorhabditis elegans (C. elegans) as a well‐established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high‐reproductive rate, well‐defined anatomy, and conserved genome, has made C. elegans one of the most successful multicellular model organisms. Discoveries obtained from the C. elegans model have made great contributions to research fields such as development, aging, biophysics, immunology, and neuroscience. Because of its transparent body and giant cell size, C. elegans is also an ideal subject for high resolution and high‐throughput optical imaging and analysis. During the past decade, great advances have been made to develop biomolecule‐targeting techniques for noninvasive optical imaging. These novel technologies expanded the toolbox for qualitative and quantitative analysis of biomolecules in C. elegans. In this review, we summarize recently developed fluorescent probes or labeling techniques for visualizing biomolecules at the cellular, subcellular or molecular scale by using C. elegans as the major model organism or designed specifically for the applications in C. elegans. Combining the technological advantages of the C. elegans model with the novel fluorescent labeling techniques will provide new horizons for high‐efficiency quantitative optical analysis in live organisms.
Caenorhabditis elegans ( C. elegans ) as a well‐established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high‐reproductive rate, well‐defined anatomy, and conserved genome, has made C. elegans one of the most successful multicellular model organisms. Discoveries obtained from the C. elegans model have made great contributions to research fields such as development, aging, biophysics, immunology, and neuroscience. Because of its transparent body and giant cell size, C. elegans is also an ideal subject for high resolution and high‐throughput optical imaging and analysis. During the past decade, great advances have been made to develop biomolecule‐targeting techniques for noninvasive optical imaging. These novel technologies expanded the toolbox for qualitative and quantitative analysis of biomolecules in C. elegans . In this review, we summarize recently developed fluorescent probes or labeling techniques for visualizing biomolecules at the cellular, subcellular or molecular scale by using C. elegans as the major model organism or designed specifically for the applications in C. elegans . Combining the technological advantages of the C. elegans model with the novel fluorescent labeling techniques will provide new horizons for high‐efficiency quantitative optical analysis in live organisms.
Caenorhabditis elegans (C. elegans) as a well-established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high-reproductive rate, well-defined anatomy, and conserved genome, has made C. elegans one of the most successful multicellular model organisms. Discoveries obtained from the C. elegans model have made great contributions to research fields such as development, aging, biophysics, immunology, and neuroscience. Because of its transparent body and giant cell size, C. elegans is also an ideal subject for high resolution and high-throughput optical imaging and analysis. During the past decade, great advances have been made to develop biomolecule-targeting techniques for noninvasive optical imaging. These novel technologies expanded the toolbox for qualitative and quantitative analysis of biomolecules in C. elegans. In this review, we summarize recently developed fluorescent probes or labeling techniques for visualizing biomolecules at the cellular, subcellular or molecular scale by using C. elegans as the major model organism or designed specifically for the applications in C. elegans. Combining the technological advantages of the C. elegans model with the novel fluorescent labeling techniques will provide new horizons for high-efficiency quantitative optical analysis in live organisms.Caenorhabditis elegans (C. elegans) as a well-established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high-reproductive rate, well-defined anatomy, and conserved genome, has made C. elegans one of the most successful multicellular model organisms. Discoveries obtained from the C. elegans model have made great contributions to research fields such as development, aging, biophysics, immunology, and neuroscience. Because of its transparent body and giant cell size, C. elegans is also an ideal subject for high resolution and high-throughput optical imaging and analysis. During the past decade, great advances have been made to develop biomolecule-targeting techniques for noninvasive optical imaging. These novel technologies expanded the toolbox for qualitative and quantitative analysis of biomolecules in C. elegans. In this review, we summarize recently developed fluorescent probes or labeling techniques for visualizing biomolecules at the cellular, subcellular or molecular scale by using C. elegans as the major model organism or designed specifically for the applications in C. elegans. Combining the technological advantages of the C. elegans model with the novel fluorescent labeling techniques will provide new horizons for high-efficiency quantitative optical analysis in live organisms.
Author Xia, Chujie
Zhang, Huimin
Wang, Chunxia
Zhu, Yi
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Snippet Caenorhabditis elegans (C. elegans) as a well‐established multicellular model organism has been widely used in the biological field for half a century. Its...
Caenorhabditis elegans ( C. elegans ) as a well‐established multicellular model organism has been widely used in the biological field for half a century. Its...
Caenorhabditis elegans (C. elegans) as a well-established multicellular model organism has been widely used in the biological field for half a century. Its...
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SubjectTerms Aging
Biomolecules
Biophysics
Body size
Caenorhabditis elegans
Cell size
DNA probes
Fluorescent indicators
fluorescent probes
Genomes
Immunology
Labeling
Life cycles
Nematodes
Nervous system
Optical analysis
optical imaging
Organisms
Probes
Qualitative analysis
Quantitative analysis
Worms
Title Innovative fluorescent probes for in vivo visualization of biomolecules in living Caenorhabditis elegans
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcyto.a.24325
https://www.ncbi.nlm.nih.gov/pubmed/33638604
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