Continuous measurement of caffeine and two metabolites in blood and skeletal muscle of unrestrained adult horses by semi-automated in vivo microdialysis

• Published in: Journal of veterinary pharmacology and therapeutics Vol. 24; no. 6; pp. 405 - 414
• Main Authors: Chou, C.-C., Webb, A. I., Brown, M. P., Gronwall, R. R., Vickroy, T. W.
• Format: Journal Article
• Language: English
• Published: Oxford UK Blackwell Science Ltd 01.12.2001
• Subjects: Animals; Area Under Curve; Caffeine - blood; Caffeine - pharmacokinetics; Central Nervous System Stimulants - blood; Central Nervous System Stimulants - pharmacokinetics; Chromatography, High Pressure Liquid - veterinary; Female; Horses - metabolism; Injections, Intravenous - veterinary; Microdialysis - instrumentation; Microdialysis - standards; Microdialysis - veterinary; Muscle, Skeletal - metabolism; Reproducibility of Results; Theobromine - metabolism; Theophylline - metabolism

Concentrations of caffeine (CA) and two metabolites were measured simultaneously in venous blood and splenius muscle of adult horses using a semi‐automated in vivo microdialysis sampling technique. Dialysates from muscle and jugular vein were collected continuously for 48 h and drug levels were dete...