Comparative evaluation of compressive and flexural strength, fluoride release and bacterial adhesion of GIC modified with CPP-ACP, bioactive glass, chitosan and MDPB

• Published in: Journal of dental research, dental clinics, dental prospects Vol. 15; no. 1; pp. 16 - 21
• Main Authors: Kirthika, Natarajan, Vidhya, Sampath, Sujatha, Venkatappan, Mahalaxmi, Sekar, Senthil Kumar, Renganathan
• Format: Journal Article
• Language: English
• Published: Iran Tabriz University of Medical Sciences 2021
• Subjects: Bacteria; bacterial adhesion; bioactive glass; Biological activity; Calcium phosphates; Cement; Chitosan; Chloride; Composite materials; cpp-acp; fluoride release; Fluorides; glass-ionomer cement; mdpb; Mechanical properties; Original; Porosity; Resins; Stainless steel; Streptococcus infections; United States > US; Mumbai India; India; Bioactive glass; Glass-ionomer cement; CPP-ACP; Bacterial adhesion; Chitosan; MDPB; Fluoride release
• DOI: 10.34172/joddd.2021.004
• ISSN: 2008-210X

Background. This study evaluated the incorporation of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), calcium sodium phosphosilicate bioactive glass (BAG), chitosan (CH), and methacryloyloxydodecylpyridinium bromide (MDPB) on the compressive and flexural strength, fluoride (F‒ ) release, and bacterial adhesion of conventional glass-ionomer cement (C-GIC). Methods. Modifications were implemented by adding CPP-ACP, BAG, and CH to the glass powder, while MDPB-GIC was prepared by incorporating MDPB to the liquid of C-GIC. Custom-made molds were used for specimen preparation. Compressive and flexural strengths were evaluated using a universal testing machine. F‒ release was calculated with Erichrome cyanide reagent, using UV-spectrophotometry, at two time intervals of 24 hours and seven days. For bacterial adhesion, the test specimens were exposed to the bacterial suspension of Streptococcus mutans and Lactobacillus acidophilus for 4 hours, and the adherent bacteria were quantified using colorimetry as the optical density (OD). Results. The incorporation of MDPB increased the flexural strength of C-GIC, with no effect on its compressive strength. CH significantly improved the compressive and flexural strength; modifications with CPP-ACP, BAG, and MDPB significantly improved the flexural strength of C-GIC. While MDPB-GIC released significantly higher F‒ at 24 hours, CPP-ACP- and BAG-modified GICs were comparable to C-GIC on day 7. C-GIC exhibited the highest bacterial adhesion, and MDPB-GIC showed the least. The data were analyzed with one-way (ANOVA), and pairwise comparisons were made with Tukey HSD tests. Conclusion. Hence, it can be concluded that the incorporation of CPP-ACP, BAG, and CH improved the mechanical properties of C-GIC, whereas MDPB improved the resistance of C-GIC to bacterial adhesion.