The effect of amygdala kindling on hippocampal neurogenesis coincides with decreased reelin and DISC1 expression in the adult dentate gyrus
Temporal lobe seizures can induce the proliferation and abnormal migration of newly generated dentate granule cells, but little is known about the molecular mechanisms that govern these pathological events. Reelin and DISC1 (disrupted‐in‐schizophrenia 1) are proteins that play a regulatory role in t...
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Published in | Hippocampus Vol. 20; no. 5; pp. 659 - 671 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.05.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Temporal lobe seizures can induce the proliferation and abnormal migration of newly generated dentate granule cells, but little is known about the molecular mechanisms that govern these pathological events. Reelin and DISC1 (disrupted‐in‐schizophrenia 1) are proteins that play a regulatory role in the maturation and integration of new neurons in the developing and adult brain. In this study, we examined whether amygdala kindling results in aberrant neurogenesis and altered expression of reelin and DISC1 in the adult dentate gyrus. Using doublecortin immunohistochemistry, we found that short‐term kindling (i.e., 30 electrical stimulations) significantly increased the number of immature neurons in the dentate subgranular zone (SGZ), whereas long‐term kindling (i.e., 99 electrical stimulations) did not. However, doublecortin‐labeled neurons in long‐term kindled rats showed greater dendritic complexity than they did in short‐term kindled or control rats. We also found that long‐term kindling decreased the number of reelin‐positive cells and decreased DISC1 expression in the dentate granule cell layer and subgranular zone. Interestingly, kindling‐induced changes in reelin and DISC1 expression coincided with the appearance of ectopically located Prox1‐labeled granule cells in the hilus. These effects occurred independently of alterations in granule cell layer length, dentate volume, or the number of hilar neurons. Taken together, these findings suggest a novel role for DISC1 in the pathophysiology of temporal lobe epilepsy and further suggest that changes in reelin and DISC1 expression may contribute to aberrant neurogenesis in the kindling model. © 2009 Wiley‐Liss, Inc. |
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Bibliography: | ark:/67375/WNG-XR1PVQTQ-4 Natural Sciences and Engineering Council of Canada (NSERC) istex:B8A72848ECC3879FB55E8663B88EEC48F87C1517 ArticleID:HIPO20653 Xunta de Galicia and Spanish Ministry for Science and Technology ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1050-9631 1098-1063 |
DOI: | 10.1002/hipo.20653 |