Explanation of inconsistencies in the determination of human serum albumin thermal stability

• Published in: International journal of biological macromolecules Vol. 232; p. 123379
• Main Authors: Nemergut, Michal, Sedláková, Dagmar, Fabriciová, Gabriela, Belej, Dominik, Jancura, Daniel, Sedlák, Erik
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.03.2023
• Subjects: calorimetry; Calorimetry, Differential Scanning; denaturation; human serum albumin; Humans; Lumry-Eyring model; Phase diagram method; Protein Denaturation; Protein thermal stability; Serum albumin; Serum Albumin, Human; spectroscopy; thermal stability; Thermodynamic and kinetic stability; Thermodynamics; Thermodynamic and kinetic stability; Protein thermal stability; Serum albumin; Phase diagram method; Lumry-Eyring model

Thermal denaturation of human serum albumin has been the subject of many studies in recent decades, but the results of these studies are often conflicting and inconclusive. To clarify this, we combined different spectroscopic and calorimetric techniques and performed an in-depth analysis of the stru...