Conformational change of lysozyme on the interaction with gene carrier polyethyleneimine

• Published in: International journal of biological macromolecules Vol. 117; pp. 532 - 537
• Main Authors: Wang, Fan, Wang, Xu, Zhang, Min, Huang, Aimin, Ma, Lin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2018
• Subjects: absorption; calorimetry; circular dichroism spectroscopy; Conformation; cytotoxicity; fluorescence; gene transfer; genes; hydrogen bonding; hydrophilicity; hydrophobic bonding; hydrophobicity; Interaction; Lysozyme; moieties; molecular weight; Polyethyleneimine; titration; Polyethyleneimine; Interaction; Lysozyme; Conformation

Polyethyleneimine (PEI) has been considered as “golden standard” for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetr...