Conformational change of lysozyme on the interaction with gene carrier polyethyleneimine
Polyethyleneimine (PEI) has been considered as “golden standard” for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetr...
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| Published in | International journal of biological macromolecules Vol. 117; pp. 532 - 537 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier B.V
01.10.2018
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| Abstract | Polyethyleneimine (PEI) has been considered as “golden standard” for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetry were conducted to investigate the interaction between PEI (average molecular weight 25,000 Da) and lysozyme and its influence on the conformation of the enzyme. PEI is an amphiphilic polymer with strong hydrogen bonding capability and weak hydrophobicity due to dense amine groups and methyl groups. However, the hydropathic characteristics that hydrophobic methyl groups and hydrophilic amine groups distributed alternatively along the polymer chain made it difficult to penetrate into the hydrophobic core, but subject to binding onto the surface of lysozyme via hydrophobic interaction and hydrogen bond, leading to a more compact conformation and an increased surface hydrophobicity of the enzyme.
•Complex formation occurred between PEI and lysozyme.•PEI binding dominated by hydrophobic interaction and hydrogen bond•PEI binding induced a more ordered conformation of lysozyme.•Surface hydrophobicity of lysozyme increased due to the interaction with PEI. |
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| AbstractList | Polyethyleneimine (PEI) has been considered as “golden standard” for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetry were conducted to investigate the interaction between PEI (average molecular weight 25,000 Da) and lysozyme and its influence on the conformation of the enzyme. PEI is an amphiphilic polymer with strong hydrogen bonding capability and weak hydrophobicity due to dense amine groups and methyl groups. However, the hydropathic characteristics that hydrophobic methyl groups and hydrophilic amine groups distributed alternatively along the polymer chain made it difficult to penetrate into the hydrophobic core, but subject to binding onto the surface of lysozyme via hydrophobic interaction and hydrogen bond, leading to a more compact conformation and an increased surface hydrophobicity of the enzyme. Polyethyleneimine (PEI) has been considered as "golden standard" for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetry were conducted to investigate the interaction between PEI (average molecular weight 25,000 Da) and lysozyme and its influence on the conformation of the enzyme. PEI is an amphiphilic polymer with strong hydrogen bonding capability and weak hydrophobicity due to dense amine groups and methyl groups. However, the hydropathic characteristics that hydrophobic methyl groups and hydrophilic amine groups distributed alternatively along the polymer chain made it difficult to penetrate into the hydrophobic core, but subject to binding onto the surface of lysozyme via hydrophobic interaction and hydrogen bond, leading to a more compact conformation and an increased surface hydrophobicity of the enzyme.Polyethyleneimine (PEI) has been considered as "golden standard" for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetry were conducted to investigate the interaction between PEI (average molecular weight 25,000 Da) and lysozyme and its influence on the conformation of the enzyme. PEI is an amphiphilic polymer with strong hydrogen bonding capability and weak hydrophobicity due to dense amine groups and methyl groups. However, the hydropathic characteristics that hydrophobic methyl groups and hydrophilic amine groups distributed alternatively along the polymer chain made it difficult to penetrate into the hydrophobic core, but subject to binding onto the surface of lysozyme via hydrophobic interaction and hydrogen bond, leading to a more compact conformation and an increased surface hydrophobicity of the enzyme. Polyethyleneimine (PEI) has been considered as “golden standard” for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the molecular basis of PEI cytotoxicity, absorption, resonance Rayleigh scattering, fluorescence, circular dichroism and isothermal titration calorimetry were conducted to investigate the interaction between PEI (average molecular weight 25,000 Da) and lysozyme and its influence on the conformation of the enzyme. PEI is an amphiphilic polymer with strong hydrogen bonding capability and weak hydrophobicity due to dense amine groups and methyl groups. However, the hydropathic characteristics that hydrophobic methyl groups and hydrophilic amine groups distributed alternatively along the polymer chain made it difficult to penetrate into the hydrophobic core, but subject to binding onto the surface of lysozyme via hydrophobic interaction and hydrogen bond, leading to a more compact conformation and an increased surface hydrophobicity of the enzyme. •Complex formation occurred between PEI and lysozyme.•PEI binding dominated by hydrophobic interaction and hydrogen bond•PEI binding induced a more ordered conformation of lysozyme.•Surface hydrophobicity of lysozyme increased due to the interaction with PEI. |
| Author | Ma, Lin Huang, Aimin Wang, Xu Wang, Fan Zhang, Min |
| Author_xml | – sequence: 1 givenname: Fan surname: Wang fullname: Wang, Fan organization: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China – sequence: 2 givenname: Xu surname: Wang fullname: Wang, Xu organization: Institute of Laboratory Medicine, Hangzhou Medical College, Hangzhou 310053, China – sequence: 3 givenname: Min surname: Zhang fullname: Zhang, Min organization: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China – sequence: 4 givenname: Aimin surname: Huang fullname: Huang, Aimin email: ham2003@163.com organization: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China – sequence: 5 givenname: Lin surname: Ma fullname: Ma, Lin email: malinzju@163.com organization: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China |
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| Snippet | Polyethyleneimine (PEI) has been considered as “golden standard” for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the... Polyethyleneimine (PEI) has been considered as "golden standard" for polymeric gene delivery carriers, however also induces cytotoxicity. To reveal the... |
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| SubjectTerms | absorption calorimetry circular dichroism spectroscopy Conformation cytotoxicity fluorescence gene transfer genes hydrogen bonding hydrophilicity hydrophobic bonding hydrophobicity Interaction Lysozyme moieties molecular weight Polyethyleneimine titration |
| Title | Conformational change of lysozyme on the interaction with gene carrier polyethyleneimine |
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