VeroNectin-4 is a highly sensitive cell line that can be used for the isolation and titration of Peste des Petits Ruminants virus

• Published in: Journal of virological methods Vol. 228; pp. 135 - 139
• Main Authors: Fakri, F., Elarkam, A., Daouam, S., Tadlaoui, K., Fassi-Fihri, O., Richardson, C.D., Elharrak, M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2016
• Subjects: Africa; Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell Culture Techniques; Cell Line; Cell sensitivity; Cercopithecus aethiops; Dogs; Goats; Humans; Morbillivirus; Nectin-4; Nectins; Paramyxoviridae; Peste-des-Petits-Ruminants - diagnosis; Peste-des-Petits-Ruminants - virology; Peste-des-petits-ruminants virus - growth & development; Peste-des-petits-ruminants virus - isolation & purification; Peste-des-petits-ruminants virus - physiology; PPR; PVRL4; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Receptors, Virus - genetics; Receptors, Virus - metabolism; Ruminantia; Sheep; Signaling Lymphocytic Activation Molecule Family Member 1; SLAM; Vero Cells; Virus Replication; Nectin-4; Morbillivirus; SLAM; PPR; PVRL4; Cell sensitivity
• DOI: 10.1016/j.jviromet.2015.11.017
• ISSN: 0166-0934

Peste des Petits Ruminants virus (PPRV) is a member of the Morbillivirus subgroup of the family Paramyxoviridae, and is one of the most contagious diseases of small ruminants throughout Africa and the rest of the world. Different cell lines have previously been used to isolate PPRV but with limited success. Thus, to improve the isolation of Morbilliviruses, human, canine, and goat homologues of the lymphocyte receptor signaling lymphocyte activation molecule (SLAM) have been introduced into cells that can support virus replication. However, the amino acid sequence of SLAM varies between species, and often requires adaptation of a particular virus to different versions of the receptor. The protein sequence of Nectin-4 is highly conserved between different mammals, which eliminate the need for receptor adaptation by the virus. Cell lines expressing Nectin-4 have previously been used to propagate measles and canine distemper viruses. In this study, we compared infections in Vero cells expressing canine SLAM (VeroDogSLAM) to those in Vero cells expressing Nectin-4 (VeroNectin-4), following inoculations with wild-type strains of PPRV. Virus isolation using VeroNectin-4 cells was successful with 23% of swabbed samples obtained from live infected animals, and was 89% effective using post-mortem tissues of infected sheep. By contrast, only 4.5% efficiency was observed from swab samples and 67% efficiency was obtained in virus isolation from post-mortem tissues using VeroDogSLAM cells. The average incubation period for virus recovery from post-mortem tissues was 3.4 days using VeroNectin-4 cells, compared with 5.5 days when using VeroDogSLAM cells. The virus titers of PPRV obtained from VeroNectin-4 cells were also higher than those derived from VeroDogSLAM cells. A comparison of the growth kinetics for PPRV in the two cell lines confirmed the superiority of VeroNectin-4 cells for PPR diagnostic purposes and vaccine virus titration.