Survey of natural scrapie in Japan: Analysis of RFLP types of the PrP gene and detection of PrPSc mainly in Suffolk sheep

• Published in: Journal of Veterinary Medical Science Vol. 56; no. 4; pp. 627 - 632
• Main Authors: Onodera, A. (Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)), Ikeda, T, Horiuchi, M, Ishiguro, N, Onuma, M, Hirano, N, Mikami, T, Honda, E, Hirai, K, Kai, K, Yugi, H, Muramatsu, Y, Shinagawa, M
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.1994
• Subjects: ANALYTICAL METHODS; Animals; Brain Chemistry; ENCUESTAS; ENQUETE; GENE; Gene Frequency; GENES; Haplotypes - genetics; JAPAN; JAPON; Lymph Nodes - chemistry; OVIN; OVINOS; Polymorphism, Restriction Fragment Length; PrPsc; PrPSc Proteins - genetics; PrPSc Proteins - isolation & purification; RESISTANCE AUX FACTEURS NUISIBLES; RESISTANCE TO INJURIOUS FACTORS; RESISTENCIA A AGENTES DANINOS; RFLP type; scrapie; Scrapie - genetics; SCRAPIE AGENT; SHEEP; Sheep - genetics; Spleen - chemistry; SURVEYS; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; VIRUS TEMBLADERA DE LOS OVINOS; VIRUS TREMBLANTE DU MOUTON; Japan

We examined the brains, spleens and/or lymph nodes of 197 mainly Suffolk sheep collected from Hokkaido, and the Tohoku, Kanto and Chubu districts to detect PrPsc and thus to estimate scrapie contamination in Japan. Sixteen sheep in Hokkaido and 2 sheep in other districts that were introduced from Ho...