In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish

• Published in: Journal of biomedicine & biotechnology Vol. 2012; no. 2012; pp. 1 - 7
• Main Authors: Leong, Ivone Un San, Lan, Chuan-Ching, Skinner, Jonathan R., Shelling, Andrew N., Love, Donald R.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2012; Hindawi Publishing Corporation
• Subjects: Analysis of Variance; Animals; Disease Models, Animal; Embryo, Nonmammalian; Ether-A-Go-Go Potassium Channels - genetics; Ether-A-Go-Go Potassium Channels - metabolism; Gene Knockdown Techniques - methods; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Long QT Syndrome; Mice; MicroRNAs - chemistry; MicroRNAs - genetics; RNA Interference; Zebrafish; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism

The zebrafish (Danio rerio) has become an attractive model for human disease modeling as there are a large number of orthologous genes that encode similar proteins to those found in humans. The number of tools available to manipulate the zebrafish genome is limited and many currently used techniques...