Validation of an immunoblot assay employing an objective reading system and used as a confirmatory test in equine infectious anaemia surveillance programs

• Published in: Journal of virological methods Vol. 266; pp. 77 - 88
• Main Authors: Scicluna, Maria Teresa, Autorino, Gian Luca, Cook, Sheila J., Issel, Charles J., Cook, R. Frank, Nardini, Roberto
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2019
• Subjects: agar; algorithms; Animals; Antibodies, Viral - blood; antigens; asses; blood serum; Confirmatory test; diagnostic sensitivity; digital images; Digital imaging; Enzyme-Linked Immunosorbent Assay; Epidemiological Monitoring - veterinary; Equine infectious anaemia; equine infectious anemia; Equine Infectious Anemia - blood; Equine Infectious Anemia - diagnosis; financial economics; gels; horses; Horses - virology; Image Processing, Computer-Assisted; Immunoblot; Immunoblotting - standards; immunodiffusion tests; Infectious Anemia Virus, Equine - immunology; Italy; monitoring; mules; notifiable disease; OIE; pet ownership; Reproducibility of Results; risk reduction; Sensitivity and Specificity; Validation; viruses; Italy; AGIDT; HRP; Equine infectious anaemia; NRL; Confirmatory test; JPEG; AUC; EDse; SD; SE; U; Dse; IB; EDsp; EIA; Dsp; LOD; PBS; R2; Validation; c-ELISA; ISO; CI; ROC; TMB; CL; OIE; RGB; FEK; Mab; Immunoblot; CV; Digital imaging; HIV; EIAV; HCV; NCS

Equine infectious anaemia (EIA) is a blood borne disease that is listed among the notifiable diseases of the World Organisation for Animal Health (OIE). EIA is also regulated by the OIE for the international trading provisions and is generally subject to control programmes. Since 2011, Italy has bee...