Properties comparison between free and immobilized wheat esterase using glass fiber film
In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carr...
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Published in | International journal of biological macromolecules Vol. 125; pp. 87 - 91 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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Netherlands
Elsevier B.V
15.03.2019
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Online Access | Get full text |
ISSN | 0141-8130 1879-0003 1879-0003 |
DOI | 10.1016/j.ijbiomac.2018.12.055 |
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Abstract | In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its Km increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application.
[Display omitted]
•The wheat esterase was immobilized on glass fiber film for the first time.•Enzymatic properties of immobilized wheat esterase are proposed.•Catalytic properties of wheat esterase changes of the presence of carrier.•Immobilized wheat esterase presenting improved characteristic than that of free enzyme. |
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AbstractList | In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its Km increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application.In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its Km increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application. In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its K increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application. In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its Km increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application. [Display omitted] •The wheat esterase was immobilized on glass fiber film for the first time.•Enzymatic properties of immobilized wheat esterase are proposed.•Catalytic properties of wheat esterase changes of the presence of carrier.•Immobilized wheat esterase presenting improved characteristic than that of free enzyme. In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively. As for optimum temperature for free and immobilized enzymes were observed to be 30 °C and 40 °C, respectively. When the enzyme was immobilized on glass fibre membranes, its Km increased about 7 times. In addition, storage and thermal stability of the free wheat esterase were increased by as a result of membrane immobilization, after 12 days of storage, the immobilized enzyme still retained about 91.10% of its original activity at 4 °C, indicating a great potential in industrial application. |
Author | Li, Shan-Shan Pu, Biao Liu, Xiang Li, Mei-Liang Chen, An-Jun Qin, Wen Zhang, Zhi-Qing Wu, He-Jun Ye, Lin Shen, Guang-Hui Liu, Xing-Yan Luo, Qing-Ying |
Author_xml | – sequence: 1 givenname: Lin surname: Ye fullname: Ye, Lin organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 2 givenname: Xiang surname: Liu fullname: Liu, Xiang organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 3 givenname: Guang-Hui surname: Shen fullname: Shen, Guang-Hui organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 4 givenname: Shan-Shan surname: Li fullname: Li, Shan-Shan organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 5 givenname: Qing-Ying surname: Luo fullname: Luo, Qing-Ying organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 6 givenname: He-Jun surname: Wu fullname: Wu, He-Jun organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 7 givenname: An-Jun surname: Chen fullname: Chen, An-Jun organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 8 givenname: Xing-Yan surname: Liu fullname: Liu, Xing-Yan organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 9 givenname: Mei-Liang surname: Li fullname: Li, Mei-Liang organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 10 givenname: Biao orcidid: 0000-0002-7840-2865 surname: Pu fullname: Pu, Biao organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 11 givenname: Wen surname: Qin fullname: Qin, Wen organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China – sequence: 12 givenname: Zhi-Qing surname: Zhang fullname: Zhang, Zhi-Qing email: zqzhang721@163.com organization: College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, PR China |
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Keywords | Characterization Immobilization Fibre membrane Plant esterase (PE) Wheat bran |
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Snippet | In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the... |
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SubjectTerms | catalytic activity Characterization esterases Fibre membrane glass fibers Immobilization immobilized enzymes industrial applications phosphates Plant esterase (PE) storage time temperature thermal stability wheat Wheat bran |
Title | Properties comparison between free and immobilized wheat esterase using glass fiber film |
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