MSPD METHOD FOR THE DETERMINATION OF CLOFENTEZINE, FLUMETRALIN, AND KRESOXIM-METHYL RESIDUES IN FRESH AND DEHYDRATED FORMS OF PLANTAGO MAJOR MEDICINAL PLANT BY HPLC/UV-DAD

• Published in: Journal of liquid chromatography & related technologies Vol. 36; no. 13; pp. 1802 - 1809
• Main Authors: dos Santos, Érica Silvia, Navickiene, Sandro, Wanderley, Kaline A., de Sá, Gilberto F., Júnior, Severino A.
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Group 01.05.2013; Taylor & Francis Ltd
• Subjects: Aluminum oxide; Arrays; Dehydration; Dichloromethane; Dispersions; Liquid chromatography; matrix solid-phase dispersion; medicinal plant; metal organic framework; pesticides; Plantago major; Silica gel; Sorbents
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826076.2012.698681

A method was developed using matrix solid-phase dispersion (MSPD), together with liquid chromatography with ultraviolet diode array detector for determination of clofentezine, flumetralin, and kresoxim-methyl in fresh and dehydrated Plantago major medicinal plant material. Parameters evaluated included the type of sorbent (C 18 -bonded silica, neutral alumina, silica gel, Florisil, and MIL-53) using dichloromethane as the eluting solvent. The best results were obtained using 0.5 g of plant material, 0.5 g of silica gel as sorbent, and dichloromethane (20 mL). The method was validated using plant samples spiked with pesticides at three concentration levels (0.5, 1.0, and 2.0 µg/g). Average recoveries (using four replicates) ranged from 62.2% to 110%, with relative standard deviations between 5.4% and 18.5%. Detection and quantification limits were in the ranges 0.05-0.07 µg/g and 0.15-0.25 µg/g, respectively.