Validating reference genes within a mouse model system of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxicity

• Published in: Chemico-biological interactions Vol. 205; no. 1; pp. 63 - 71
• Main Authors: Prokopec, Stephenie D, Buchner, Nicholas B, Fox, Natalie S, Chong, Lauren C, Mak, Denise Y.F, Watson, John D, Petronis, Arturas, Pohjanvirta, Raimo, Boutros, Paul C
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 05.09.2013
• Subjects: Algorithms; animal models; Animals; data collection; essential genes; Female; Gene Expression - drug effects; Genes, Essential - drug effects; genotype; Hypoxanthine Phosphoribosyltransferase - genetics; Male; messenger RNA; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, Transgenic; Multivariate Analysis; Peptide Elongation Factor 1 - genetics; Polychlorinated Dibenzodioxins - toxicity; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; tetrachlorodibenzo-p-dioxin; toxicity; toxins; variance; Quantitative real-time PCR; C(q); Reference genes; TCDD; ARNT; AHR; 2,3,7,8-tetrachlorodibenzo-p-dioxin; quantification cycle; aryl hydrocarbon receptor; normalized expression level; aryl hydrocarbon receptor nuclear translocator; NEL; mRNA abundance; Mouse model; Normalization; qPCR

BACKGROUND: Quantitative real-time PCR (qPCR) is the “gold-standard” technique for measuring mRNA abundances. To correctly compare samples and generate biologically valid results, qPCR data usually require comprehensive normalization to account for sample content variation between reactions. The mos...