Preparation, characterization and antiglycation activities of the novel polysaccharides from Boletus snicus

• Published in: International journal of biological macromolecules Vol. 92; pp. 607 - 614
• Main Authors: Liping, Sun, Xuejiao, Su, Yongliang, Zhuang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2016
• Subjects: advanced glycation end products; Antiglycation activities; arabinose; Basidiomycota - chemistry; Boletus; Carbon-13 Magnetic Resonance Spectroscopy; Chemical characterization; chromatography; dose response; Fourier transform infrared spectroscopy; galactose; glucosamine; glucose; glucuronic acid; glycation; Glycation End Products, Advanced; Glycosylation - drug effects; mannose; Molecular Weight; Monosaccharides - analysis; mushrooms; Nitroblue Tetrazolium; nuclear magnetic resonance spectroscopy; Polysaccharides; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Proton Magnetic Resonance Spectroscopy; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Antiglycation activities; Polysaccharides; Chemical characterization
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.07.014

Boletus snicus (BS) is one of the commercially important mushroom species. Two polysaccharides (BSP-1b and BSP-2b) were extracted and purified from the body of BS by DEAE-cellulose and Sephadex G-100 column chromatography. The average of molecular weight of BSP-1b and BSP-2b were 59.21kDa and 128.74kDa. BSP-1b is a heteropolysaccharide with a large number of glucose and a small amount of mannose, glucosamine hydrochloride and arabinose. The monosaccharide compositions of BSP-2b contain mannose, glucuronic acid, glucosamine hydrochloride, glucose, galactose, arabinose with the molar ratio of 10.70:6.95:12.05:12.57:1.83:1.00. The FTIR spectra and NMR analysis demonstrated that BSP-1b and BSP-2b existed pyranose ring structure and BSP-2b had high content of uronic acid. The antiglycation activities of BSP-1b and BSP-2b were investigated. The results showed BSP-1b and BSP-2b had high inhibitory effects on glycation and exhibited dose-dependent responses. BSP-2b showed stronger antiglycation activity than BSP-1b. This study indicated that the BSP-2b could effectively inhibit the formation of advanced glycation end-products.