Comprehensive measurements of salivary pellicle thickness formed at different intraoral sites on Si wafers and bovine enamel

[Display omitted] •First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A physiologically important rapid initial pellicle formation stage was detected.•Inter-individual and formation kinetics differences were found...

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Published inColloids and surfaces, B, Biointerfaces Vol. 174; pp. 246 - 251
Main Authors Güth-Thiel, Sabine, Kraus-Kuleszka, Ines, Mantz, Hubert, Hoth-Hannig, Wiebke, Hähl, Hendrik, Dudek, Johanna, Jacobs, Karin, Hannig, Matthias
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.02.2019
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Abstract [Display omitted] •First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A physiologically important rapid initial pellicle formation stage was detected.•Inter-individual and formation kinetics differences were found.•Oral location had a lower impact on pellicle thickness than individual influence. The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies.
AbstractList The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies.The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies.
The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies.
[Display omitted] •First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A physiologically important rapid initial pellicle formation stage was detected.•Inter-individual and formation kinetics differences were found.•Oral location had a lower impact on pellicle thickness than individual influence. The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies.
Author Dudek, Johanna
Jacobs, Karin
Hähl, Hendrik
Güth-Thiel, Sabine
Mantz, Hubert
Hoth-Hannig, Wiebke
Kraus-Kuleszka, Ines
Hannig, Matthias
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Keywords Thickness
Transmission electron microscopy
Salivary pellicle
Kinetics
In situ
Ellipsometry
Language English
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Snippet [Display omitted] •First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A...
The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a...
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SubjectTerms adsorption
Adult
Animals
Cattle
colloids
Dental Enamel - chemistry
Dental Pellicle - chemistry
Ellipsometry
enamel
Female
Humans
In situ
Kinetics
Male
oral health
Saliva - chemistry
Salivary pellicle
silicon
Silicon Compounds - chemistry
therapeutics
Thickness
Transmission electron microscopy
wafers
Title Comprehensive measurements of salivary pellicle thickness formed at different intraoral sites on Si wafers and bovine enamel
URI https://dx.doi.org/10.1016/j.colsurfb.2018.11.020
https://www.ncbi.nlm.nih.gov/pubmed/30469045
https://www.proquest.com/docview/2137460782
https://www.proquest.com/docview/2220859396
Volume 174
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