Comprehensive measurements of salivary pellicle thickness formed at different intraoral sites on Si wafers and bovine enamel
[Display omitted] •First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A physiologically important rapid initial pellicle formation stage was detected.•Inter-individual and formation kinetics differences were found...
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Published in | Colloids and surfaces, B, Biointerfaces Vol. 174; pp. 246 - 251 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
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Netherlands
Elsevier B.V
01.02.2019
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Abstract | [Display omitted]
•First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A physiologically important rapid initial pellicle formation stage was detected.•Inter-individual and formation kinetics differences were found.•Oral location had a lower impact on pellicle thickness than individual influence.
The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies. |
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AbstractList | The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies.The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies. The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies. [Display omitted] •First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A physiologically important rapid initial pellicle formation stage was detected.•Inter-individual and formation kinetics differences were found.•Oral location had a lower impact on pellicle thickness than individual influence. The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a protective layer in the maintenance of oral health. Pellicle thickness measurements are a central tool helping to understand how exogenous manipulations may influence pellicle formation. This is of particular importance for the investigation of new preventive and therapeutic approaches. In the present study we determined the kinetics of the in situ pellicle thickness formation at different intraoral sites and investigated how pellicle formation occurs in different individuals. To address the kinetic aspect, the thickness of the in situ pellicle was determined after formation periods of 3 min, 30 min and 120 min. The thickness of the pellicle was either measured on silicon wafers by ellipsometry or on bovine enamel by transmission electron microscopy. We found a physiologically important rapid pellicle formation phase within the first minutes and a slow pellicle formation phase between 30 min and 120 min. Furthermore, our results identify significant inter-individual differences both for the pellicle thickness and for the formation kinetics, indicating the consideration of individual-specific differences of the pellicle layer as an important aspect for future studies. |
Author | Dudek, Johanna Jacobs, Karin Hähl, Hendrik Güth-Thiel, Sabine Mantz, Hubert Hoth-Hannig, Wiebke Kraus-Kuleszka, Ines Hannig, Matthias |
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Keywords | Thickness Transmission electron microscopy Salivary pellicle Kinetics In situ Ellipsometry |
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•First systematic examination of the in situ pellicle thickness.•Ellipsometry is a powerful method for measurements of pellicle thickness.•A... The salivary pellicle is a thin acellular film formed on orally exposed surfaces by adsorption of macromolecules from the oral fluids and serves as a... |
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SubjectTerms | adsorption Adult Animals Cattle colloids Dental Enamel - chemistry Dental Pellicle - chemistry Ellipsometry enamel Female Humans In situ Kinetics Male oral health Saliva - chemistry Salivary pellicle silicon Silicon Compounds - chemistry therapeutics Thickness Transmission electron microscopy wafers |
Title | Comprehensive measurements of salivary pellicle thickness formed at different intraoral sites on Si wafers and bovine enamel |
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