Optimization of Culture Conditions for 1,3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae Using Response Surface Methodology

• Published in: Biotechnology and bioprocess engineering Vol. 13; no. 6; pp. 666 - 670
• Main Authors: Oh, B.R. (Molecular Bioprocess Research Centre, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea), Seo, J.W. (Molecular Bioprocess Research Centre, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea), Choi, M.H. (Molecular Bioprocess Research Centre, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea), Kim, C.H. (Molecular Bioprocess Research Centre, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea), E-mail: kim3641@kribb.re.kr
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.12.2008; Springer Nature B.V; 한국생물공학회
• Subjects: 1,3-propanediol; Biodiesel fuels; Biotechnology; central composite design; Chemistry; Chemistry and Materials Science; crude glycerol; Cultivation; Experiments; Glycerol; Independent variables; Industrial and Production Engineering; KLEBSIELLA PNEUMONIAE; Optimization; response surface methodology; Statistical analysis; Statistical models; Studies; Variables; Variance analysis; 생물공학; Klebsiella pneumoniae
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-008-0090-8

To produce 1,3-propanediol (1,3-PD) from crude glycerol, cultivation conditions were optimized by response surface methodology (RSM) based on a 2∨5 factorial central composite design (CCD). RSM was adopted to derive a statistical model for the individual and interactive effects of crude glycerol, (NH₄)₂SO₄, pH, cultivation time and temperature on the production of 1,3-PD. Optimal conditions for maximum 1,3-PD production were as follows: crude glycerol, 35 g/L; (NH₄)₂SO₄, 8 g/L; pH, 7.37; cultivation time, 10.8 h; temperature, 36.88℃. Under these optimal conditions, the design expert presented the maximal numerical solution with a predicted 1,3-PD production level of up to 13.74 g/L. The experimental production of 1,3-PD yielded 13.8 g/L, which was in close agreement with the model prediction.