Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2

• Published in: Plant science (Limerick) Vol. 304; p. 110825
• Main Authors: Li, Jinjin, Wang, Weiwei, Yuan, Jinhong, Xu, Jinyu, He, Lifei, Zhang, Xinying, Zhang, Haiyan
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.03.2021
• Subjects: Arabidopsis; Arabidopsis - metabolism; Arabidopsis - ultrastructure; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Cell Membrane - metabolism; Cell Membrane - ultrastructure; confocal microscopy; copper; Copper - metabolism; Copper Transport Proteins - metabolism; COPT2; degradation; endocytosis; Endoplasmic Reticulum - metabolism; Endoplasmic Reticulum - ultrastructure; H-transporting ATP synthase; lysine; Microscopy, Confocal; nutrient uptake; nutrients; plasma membrane; Polymerase Chain Reaction; Proteasome; proteasome endopeptidase complex; Proteasome Endopeptidase Complex - metabolism; proteinases; site-directed mutagenesis; SLC31 Proteins - metabolism; soil; transcription (genetics); transporters; Ubiquitin; Ubiquitin - metabolism; Ubiquitination; Vacuolar degradation; vacuoles; Vesicle trafficking; Ubiquitin; Vesicle trafficking; Vacuolar degradation; Arabidopsis; COPT2; Proteasome
• ISSN: 0168-9452; 1873-2259; 1873-2259
• DOI: 10.1016/j.plantsci.2021.110825

•COPT2 is localized at the plasma membrane and endoplasmic reticulum.•COPT2 protein is downregulated in response to excess copper.•Turnover of COPT2 is controlled by ubiquitin-independent proteasomal degradation.•C-terminal lysine residues of COPT2 is required for copper acquisition but not for COPT...