Marinobacterium boryeongense sp. nov., isolated from seawater

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 2; pp. 493 - 497
• Main Authors: Kang, Ji Young, Kim, Mi-Jung, Chun, Jeesun, Son, Kyung Pyo, Jahng, Kwang Yeop
• Format: Journal Article
• Language: English
• Published: England 01.02.2019
• Subjects: Marinobacterium boryeongense sp. nov; Poly-3-hydoxybutyrate
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003184

A Gram-stain-negative and strictly aerobic bacterium, designated DMHB-2 , was isolated from a sample of seawater collected off the Yellow Sea coast of the Republic of Korea. Cells were short rods and motile by means of a single polar flagellum. Catalase and oxidase activities were positive. Growth occurred at pH 5.5-10.0 (optimum, pH 6.0), 15-45 °C (optimum, 25 °C) and with 1-9 % NaCl (optimum, 3 %). The respiratory quinone was ubiquinone-8 and the major fatty acids were C16 : 0 (17.9 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 26.1 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 37.4 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DMHB-2 belong to the genus Marinobacterium, with the highest 16S rRNA gene sequence similarity of 95.2 % to Marinobacterium zhoushanense KCTC 42782 . The genomic DNA G+C content of strain DMHB-2 was 60.8 mol%. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics presented in this study, strain DMHB-2 is suggested to represent a novel species of the genus Marinobacterium, for which the name Marinobacteriumboryeongense sp. nov. is proposed. The type strain is DMHB-2 (=KACC 19225 =JCM 31902 ).