A short review of applications of liquid chromatography mass spectrometry based metabolomics techniques to the analysis of human urine

The applications of metabolomics as a methodology for providing better treatment and understanding human disease continue to expand rapidly. In this review, covering the last two years, the focus is on liquid chromatography-mass spectrometry (LC-MS) profiling of metabolites in urine. In LC-MS based...

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Bibliographic Details
Published inAnalyst (London) Vol. 14; no. 9; pp. 297 - 2915
Main Authors Zhang, Tong, Watson, David G
Format Journal Article
LanguageEnglish
Published England 07.05.2015
Subjects
Biomarkers - urine
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Humans
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Metabolomics - instrumentation
Metabolomics - methods
Urinalysis - instrumentation
Urinalysis - methods
Urine - chemistry
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Summary:The applications of metabolomics as a methodology for providing better treatment and understanding human disease continue to expand rapidly. In this review, covering the last two years, the focus is on liquid chromatography-mass spectrometry (LC-MS) profiling of metabolites in urine. In LC-MS based metabolomics there are still problems with regard to: chromatographic separation, peak picking and alignment, metabolite identification, metabolite coverage, instrument sensitivity and data interpretation and in the case of urine sample normalisation. Progress has been made with regard to all of these issues during the period of the review. Of particular interest are the increasing use of orthogonal chromatographic methods for optimal metabolite coverage and the increasing adoption of receiver operator characteristic (ROC) curves for biomarker validation. Mass spectrometry based metabolomics profiling.
Bibliography:David Watson is a reader in pharmaceutical sciences at the University of Strathclyde. To date he has supervised 38 PhD students to completion. He has published 220 papers and reviews on a variety of topics including chromatography, bioanalysis, metabolomics and natural products. He has also published two undergraduate textbooks on pharmaceutical analysis and pharmaceutical chemistry. In the past ten years he has specialised in mass spectrometry based metabolomics and has learned a lot about metabolic pathways. He was organiser and local chair for Metabolomics 2013.
Tong Zhang received his PhD in Pharmaceutical Analysis in 2008 from the University of Strathclyde. Since 2009 he has been working as a MS/NMR research technologist in Strathclyde Institute of Pharmacy and Biological Sciences (SIPBS). His research interests focus on development and application of hyphenation techniques (LC-MS, LC-MS/MS, GC-MS and LC-UV-ELSD) in analysis of complex samples and the relevant data processing/interpretation using chemometrics methods.
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ISSN:0003-2654
1364-5528
DOI:10.1039/c4an02294g