A simple and high efficiency purification of His-tagged turnip yellow mosaic virus-like particle (TYMV-VLP) by nickel ion affinity precipitation - Tan, Foo Hou; Ng, Jeck Fei; Mohamed Alitheen, Noorjahan Banu; Muhamad, Azira; Yong, Chean Yeah; Lee, Khai Wooi (Journal of virological methods, 2023)
Keywords: affinity chromatography; chlorides; coat proteins; dialysis; Escherichia coli; immunogenicity; nanobiotechnology; nickel; Nickel affinity precipitation; Poly-histidine tagged protein; sucrose; transmission electron microscopy; Turnip yellow mosaic virus; Turnip yellow mosaic virus (TYMV); turnips; TYMV coat protein; TYMVc; ultracentrifugation; vaccine development; viruses; VLP purification; Poly-histidine tagged protein; TYMVc; VLP purification; TYMV coat protein; Turnip yellow mosaic virus (TYMV); Nickel affinity precipitation