Development and validation of a hydrophilic interaction liquid chromatography‐tandem mass spectrometry method for the quantification of regadenoson in human plasma and its pharmacokinetic application

• Published in: Journal of separation science Vol. 45; no. 6; pp. 1146 - 1152
• Main Authors: Wang, Dun‐Jian, Wang, Da‐Wei, Fang, Qiu‐Chen, Shen, Ye, Zeng, Nv‐Jin, Yang, Yan‐Ling, Zhang, Hong‐Wen, Wang, Yong‐Qing, Sun, Lu‐Ning
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.03.2022
• Subjects: Acetonitrile; Adenosine; agonists; Ammonium acetate; Blood plasma; calibration; Cardiac stress tests; Chromatography, High Pressure Liquid; Chromatography, Liquid - methods; exercise; Humans; hydrophilic interaction liquid chromatography; hydrophilicity; Hydrophobic and Hydrophilic Interactions; Ions; Liquid chromatography; Mass spectrometry; Pharmacokinetics; Pharmacology; Purines; Pyrazoles; Radioisotopes; regadenoson, tandem mass spectrometry; Reproducibility of Results; Scientific imaging; separation; Spectroscopy; tandem mass spectrometry; Tandem Mass Spectrometry - methods; pharmacokinetics; regadenoson, tandem mass spectrometry; hydrophilic interaction liquid chromatography

Regadenoson, the first selective adenosine A2A receptor agonist, is used to perform exercise stress test during radionuclide myocardial perfusion imaging. To detect the concentration of regadenoson in human plasma, a simple, fast, and sensitive tandem mass spectrometry method was established herein....