Rhesus macaques inoculated with molecularly cloned simian immunodeficiency virus

• Published in: Journal of medical primatology Vol. 18; no. 3-4; p. 311
• Main Authors: Marthas, M L, Banapour, B, Sutjipto, S, Siegel, M E, Marx, P A, Gardner, M B, Pedersen, N C, Luciw, P A
• Format: Journal Article
• Language: English
• Published: Denmark 01.01.1989
• Subjects: Animals; Antibodies, Viral - biosynthesis; Cell Line; Cells, Cultured; Cloning, Molecular; Cytopathogenic Effect, Viral; DNA, Viral - genetics; Immunoblotting; Macaca - microbiology; Macaca mulatta - microbiology; Macrophages - microbiology; Macrophages - ultrastructure; Microscopy, Electron; Monkey Diseases - microbiology; Neutralization Tests; Proviruses - genetics; Proviruses - pathogenicity; Retroviridae Infections - microbiology; Retroviridae Infections - veterinary; Simian Immunodeficiency Virus - genetics; Simian Immunodeficiency Virus - immunology; Simian Immunodeficiency Virus - pathogenicity; Simian Immunodeficiency Virus - ultrastructure; Transfection

We have isolated a biologically active molecular clone of simian immunodeficiency virus (SIV), SIVmac 1A11, originally obtained from a rhesus macaque at the New England Regional Primate Research Center. Virus derived from cells transfected with this clone is cytopathic for rhesus peripheral blood mo...