A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component

• Published in: Wellcome open research Vol. 6; p. 3
• Main Authors: Cutts, Erin E, Taylor, Gillian C, Pardo, Mercedes, Yu, Lu, Wills, Jimi C, Choudhary, Jyoti S, Vannini, Alessandro, Wood, Andrew J
• Format: Journal Article
• Language: English
• Published: England F1000 Research Limited 2021; Wellcome
• Subjects: PBAF; antibody; condensin; BAF; SWI/SNF; specificity; SMC complex

Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-...