A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component

Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-...

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Published inWellcome open research Vol. 6; p. 3
Main Authors Cutts, Erin E, Taylor, Gillian C, Pardo, Mercedes, Yu, Lu, Wills, Jimi C, Choudhary, Jyoti S, Vannini, Alessandro, Wood, Andrew J
Format Journal Article
LanguageEnglish
Published England F1000 Research Limited 2021
Wellcome
Subjects
PBAF
antibody
condensin
BAF
SWI/SNF
specificity
SMC complex
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Summary:Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI/SNF family of chromatin remodelling complexes in an NCAPH2-independent manner. This cross-reactivity, with an abundant chromatin-associated factor, is likely to affect the interpretation of protein and chromatin immunoprecipitation experiments that make use of this antibody probe.
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No competing interests were disclosed.
ISSN:2398-502X
2398-502X
DOI:10.12688/wellcomeopenres.16482.1