The development of a next‐generation sequencing panel targeting cannabinoid synthase genes to distinguish between marijuana and hemp

• Published in: Electrophoresis Vol. 45; no. 9-10; pp. 948 - 957
• Main Authors: Cheng, Ya‐Chih, Houston, Rachel
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.05.2024
• Subjects: Biomarkers; cannabinoids; Cannabis; Cannabis sativa; Chemical composition; chemotypes; cultivars; Differentiation; electrophoresis; Flow charts; Genes; Hemp; Marijuana; next‐generation sequencing; principal component analysis; Principal components analysis; pseudogenes; synthase genes; hemp; pseudogenes; synthase genes; marijuana; next-generation sequencing

Hemp and marijuana, both derived from Cannabis sativa L. (C. sativa), are subject to divergent legal regulations due to their different Δ9‐tetrahydrocannabinol (Δ9‐THC) contents. Cannabinoid synthase genes are considered the key enzymes that determine the chemical composition or chemotype of a parti...